|
The Objective: liver qi stagnation Shugan Yiyang capsule (SGYY) Huoxuetongluo Prescription kidney vibration atrophy of efficacy mechanism , as well as for the good clinical efficacy , the choice of the appropriate animal model to study the corpora cavernosa NOS Asia the type molecule expression , in order to reveal the mechanism Shugan Yiyang capsule on the treatment of erectile dysfunction (ED) . Methods: adult male Sprague - Dawley rats, mice aged 3 months , weighing 250 -300g . Adaptability rats feeding three days later , to reference literature selection bilateral internal iliac artery ligation established vascular erectile dysfunction model . Intraperitoneal injection of 10% chloral hydrate anesthesia, skin incision abdominal incision , separation of the abdominal wall muscle tissue exposed abdominal aorta and iliac artery , common iliac artery surgery under a magnifying glass along the line carefully separated until the internal iliac artery , 8-0 line ligation of the internal iliac artery . Observed two weeks into a mold , no infection and other complications . Will become the mold after the rats were randomly divided into four groups , 10 in each group : SGYY small dose group the SGYY large dose sildenafil group , model group , another sham group . Ig : molded the SGYY small dose treatment group 0.5g/kg/d , SGYY high-dose treatment group 1g/kg/d sildenafil group 10.5mg/kg/d , model group and the sham group equal amount of distilled water , the course of 30 days . One week after the end of administration to take the corpus cavernosum tissue stored in liquid nitrogen fluorescence quantitative PCR and western blot assay of nNOS in of eNOS, iNOS molecule mRNA and protein expression . Conclusion The : quantitative PCR experimental results show that the Shugan Yiyang capsule can effectively enhance the mRNA expression of NOS isoforms , the cavernous tissue of eNOS , iNOS of nNOS mRNA expression was elevated , high-dose effect is more significant . Western Blot experimental results show that , Shugan Yiyang capsule can effectively improve the cavernous tissue nNOS, iNOS protein expression of eNOS , significantly superior to low dose and high-dose effect . In summary , this thesis is proved by experiments Shugan Yiyang capsule on arteriogenic ED treatment effect clear Shugan Yiyang capsule ED molecular mechanism by detecting eNOS, iNOS, nNOS gene expression and protein expression .
|