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The new law intervention study of ischemic myocardial apoptosis SIRT1 signal transduction mechanisms and Quyu of Health

Author: ZuoFei
Tutor: WangJie；YaoKuiWu
School: Beijing University of Traditional Chinese Medicine
Course: Chinese medical science
Keywords: Ischemic heart disease SIRT1 Of of Xuefuzhuyu capsule Apoptosis
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 56
Quote: 0
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Abstract


Background ischemic heart disease has now become one of the major disease threat posed to human health, which is characterized by a complete systolic and diastolic function irreversible myocardial cells apoptosis. Studies have shown that the pathological mechanism of SIRT1-mediated signal transduction pathway and IHD is closely related to the signal transduction pathway to prolong cell life and inhibit ischemic apoptosis through regulation of its downstream gene. Resveratrol and L-NAME its sensitizer and inhibitor. At the same time, the pathway to the treatment of ischemic heart disease mechanism and TCM Quyu the new health law happens to coincide. And the current of Xuefuzhuyu soup (This topic of Xuefuzhuyu capsule alternative) with typical application as \Therefore, Chinese medicine regulation of SIRT1 signal transduction pathway of the treatment of ischemic heart disease mechanism may open up a new field of Chinese medicine treatment of ischemic heart disease. The research purpose of to clarify the IHD lesions ischemic myocardial cells SIRT1 inhibition mediated apoptosis signal transduction mechanism, to further clarify the mechanism of IHD progress to heart failure, arrhythmias. 2, to explore of Xuefuzhuyu capsule intervention role of SIRT1-mediated inhibition of apoptosis signal transduction pathway and provide an objective basis for the clinical application of herbal compound. The 3 new the drug of Xuefuzhuyu capsule ability to further promote the use of sensitizers as the SIRT1 inhibition of cell apoptosis pathway clear Quyu Health. Provides research targets for the prevention of ischemic heart disease as well as in medicine. MATERIALS AND METHODS healthy adult Wister rats were randomly divided into 8 groups 80, that is, the normal group, sham operation group, ischemia group and blood Zhuyu, the low-dose group, the resveratrol group L-NAME group 10 in each group. In addition to the normal group, sham group, the remaining rats were prepared by ligation of the left coronary artery myocardial ischemia model, 30min after releasing the ligature. Sham group only wear lines ligation. Resveratrol group before ischemia 15min sublingual intravenous administration; L-NAME group, one day before the modeling intraperitoneal injection, 2mg / 1 times / day. The of Xuefuzhuyu, low-dose group, after the success of the model, 10,20,30 mg / kg of Xuefuzhuyu capsules were given orally, the time of 10d. The capacity of the normal group, sham operation group, ischemia group gastric feeding saline. The heart was removed after the end of the experiment, electron microscopy specimens collected and quantitative polymerase chain reaction (PCR) specimens were carried out electron microscopy, radiography, and quantitative PCR. Experimental data using the SPSS19.0 statistical package for statistical analysis. Results electron microscopy results: among the groups exist in the morphology than significant differences. 2.PCR test results: of SIRT1, P53 (NF)-kappa B, of FOXO1, FOXO3, FOXO4 expression between the normal group, sham group, the resveratrol group of Xuefuzhuyu capsule high-dose group group no statistically significant differences, (P gt; 0.05); ischemia group and between groups of L-NAME group no significant difference (P gt; 0.05); were normal group, sham group, the resveratrol group Xuefu gradually compare the group the stasis capsule high-dose group and ischemia group and L-NAME group significant difference (P gt; 0.05). Capsules in of Xuefuzhuyu dose group of Xuefuzhuyu of capsule gene expression of the low-dose group larger low-dose group, but better than the ischemic group and L-NAME group. Conclusion 1. SIRT1-mediated signal transduction pathway to effective regulation of P53 (NF)-kappa B, of FOXO1, FOXO3 and FOXO4 downstream genes, inhibiting its expression, thereby inhibiting myocardial apoptosis, reducing the number of myocardial necrosis cells improve heart function, and therefore can reduce heart failure caused by reperfusion of ischemic heart disease and cardiac arrhythmias. 2. SIRT1 signal transduction pathway the TCM \3 of Xuefuzhuyu capsule as a \Provides research targets for the prevention of ischemic heart disease as well as in medicine.

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