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Experimental Research of Wuji-Baifeng Wan on the Climacteric Syndrome of Sleep and Hormone Levels in Tested Mice

Author: QiuYongFei
Tutor: GuJiHong
School: Guangzhou University of Traditional Chinese Medicine
Course: Chinese medicine
Keywords: Wujibaifeng Pills Menopausal syndrome Sleeping E2 T LH FSH
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 83
Quote: 1
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Abstract

Purpose menopause syndrome (Climacteric Syndrome, CS) is harmful to menopause women's health, one of the major diseases affect the lives of menopausal women. Think that the CS pathogenesis of neurological, endocrine, immune three system dysfunction, In 1997 Basedovskv (NEI-N) asked the famous neuroendocrine immune network theory, the three systems constitute a complete network in the past, this point of view match the overall concept of traditional Chinese medicine. CS treatment, modern medicine, the use of hormone replacement therapy, but because of long-term medication, side effects, thus limiting its clinical application areas. Chinese medicine research in recent years to combat the disease there are a lot of reports, but to seek to both therapeutic purposes, and reducing the drug has always been the effective treatment of patients with injuries is of TCM gynecology scholars explore important issue. Wujibaifeng Pills is a common gynecological proprietary Chinese medicines, and good results for kidney cause menstrual disorders, and pathogenesis of CS medicine that is closely related with the kidney, this study from Wujibaifeng Pills menopausal syndrome the rat sleep and hormone explore Wujibaifeng Pills treatment of menopausal syndrome mechanism Wujibaifeng Pills provide experimental evidence for the treatment of menopausal syndrome, and at the same time provide a theoretical basis for the range of clinical applications for expanding Wujibaifeng Pills. Method 1. Menopause syndrome mice preparation natural aging of the 9-month-old mice, conducted mouse vaginal smear detection, the mice typical cycle changes, extension of the estrous cycle disorders or diestrus mice elected menopausal syndrome in mice. . Grouping method of administration a random basis to menopause syndrome mice were divided into control group and Wujibaifeng Pills high-dose group, middle dose group and low dose group. Dose in mice in proportion to the body surface area 1:0.0026 · 20g-1 to 1-fold amount calculation, administration of high, medium, low three groups were eight times, four times, two times the amount of drug was pulverized, dissolved in a small mice in drinking water, according to the weight 0.3m1 · 10g-1 water gavage, gavage blank control group 0.3m1 · 10g-1 drinking water intragastrically once a day for 30 days. Wujibaifeng Pills menopause syndrome mice sleep sedative-hypnotics generally able to extend the principle of sodium pentobarbital sleep time, after the end of the last gavage, pentobarbital sodium powder for injection to mice by intraperitoneal injection at 35mg · kg-1. Where 30min animals turned positive reflex more than 1min, has indicated that the occurrence of sleep. Mice the number of responses and calculated for each group of mice injected drugs sleep. 4. Wujibaifeng Pills menopause syndrome related to hormonal influences sleep test the next day, with the eyeball blood analysis experiments Kunming mice the plasma placed 2mlEP tube, about 15 minutes the room temperature 2000rmp centrifugation, the supernatant liquid at -20 mark after. C cryopreserved for future detection. By radioimmunoassay of estradiol (E2), testosterone (T), luteinizing hormone (LH), follicle-stimulating hormone (FSH) detection and T/E2 ratio analysis. Statistical Methods SPSS16.0 software package for data analysis. Results. Wujibaifeng Pills menopause syndrome mice sleep: blank control group after the injection of sodium pentobarbital, low, medium and high dose groups occurred Sleep mice were: 3, 9, 9, 11, the high dose group and the control group difference was statistically significant P lt; 0.05 2. the different doses Wujibaifeng Pills able elevated serum levels of E2, menopausal syndrome in mice, in high doses group and blank control group differences were statistically significant (P lt; 0.05). 3 different dose Wujibaifeng Pills able elevated serum levels of T-menopausal syndrome mice, in the high-dose group and blank control group differences were statistically significant (P lt; 0.05). 4 different doses Wujibaifeng Pills can reduce menopause syndrome mouse LH serum levels in the high-dose group and blank control group differences were statistically significant (P lt; 0.05). 5 different doses Wujibaifeng Pills can reduce serum levels of FSH menopausal syndrome in mice, the high dose group and the control group difference was statistically significant (P lt; 0.05). 6. Different doses Wujibaifeng Pills can reduce menopause syndrome mice T/E2 ratio is low, in the high-dose group and blank control group differences were statistically significant (P lt; 0.05). Conclusions were improved in the mice of sleep with different doses Wujibaifeng Pills gavage menopause syndrome, Wujibaifeng Pills improved by adjusting the balance between the yin and yang organs menopause syndrome sleep. Kidney and hypothalamus - pituitary - ovarian axis dysfunction is closely related to the kidney can cause hypothalamic - pituitary - ovarian axis balance destruction, the performance of a series of related hormones disorders, replenishing kidney essence, adjustment disorder hormone levels, so The balance of the hypothalamic - pituitary - ovarian axis relative recovery. Wujibaifeng Pills can increase menopausal syndrome in mice E2 T serum levels, while reducing LH, FSH serum levels, Wujibaifeng Pills can make the hypothalamic - pituitary - ovarian can restore relative balance. Wujibaifeng Pills can to reduce menopause syndrome the mice T/E2 ratio prompted Wujibaifeng Pills may have a role in delaying ovarian function decline. Wujibaifeng Pills whether to adjust other hormone levels, or to have an impact in other areas such as immunization, radicals or adrenal, need to be further explored.

Experimental Research of Wuji-Baifeng Wan on the Climacteric Syndrome of Sleep and Hormone Levels in Tested Mice

Abstract

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