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The Extraction of Volatile Oil in Anoectochilus and Its Inhibition Effect in Lung Cancer Cell NCI-H446

Author: ZuoWanCai
Tutor: HuangXian
School: Fujian Medical
Course: Pharmacy
Keywords: Anoectochilus Volatile oil Gas chromatography - mass Apoptosis NCI-H446
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 87
Quote: 1
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Abstract


Objective: This article by steam distillation extraction of rare herbs gold line lotus in the volatile oil, qualitative analysis of Anoectochilus volatile oil; research gold line lotus volatile oil of lung cancer cells in the NCI-H446 proliferation activity of, and to further explore the golden thread volatile oil components in the lotus antitumor mechanism. Research methods: the application of steam distillation, water bath reflux extraction and Soxhlet extraction, extraction volatile oil components Anoectochilus, volatile oils extraction rate indicators, examine the impact of three different extraction methods on extraction rate; gas chromatography - mass spectrometry, the extracted gold line lotus volatile oil ingredients conduct qualitative analysis; using MTT detection method, the detection of gold wire lotus volatile oil on the role of human lung cancer cells in the NCI-H446 of cytotoxic through inhibition rate curve gold line lotus volatile oil IC50; application TUNEL method, transmission electron microscopy was used to observe volatile oil Anoectochilus morphological changes of tumor cells to promote apoptosis; volatile oil in the application of DNA-ladder Anoectochilus qualitative analysis of tumor cell apoptosis. Results: The volatile oils extracted by steam distillation, extraction rate was 0.06%, higher than the other two methods. Compared to the other two volatile oil extraction method, steam distillation consuming short, the advantages of organic solvents with less. Gas chromatography - mass spectrometry volatile oil components of the herbal medicines Anoectochilus exist qualitative analysis, a total of 78 compounds can be detected, using computer mass spectra of each peak NIST standard mass retrieval database search and Mass Spectrometry law check , 37 compounds were identified. Using the area normalization method to calculate the content of each compound, accounting for 96.26% of the total volatile oil. The volatile oil components to 16 carboxylate (47.98%), palmitic acid (20.57%). The volatile oil Anoectochilus inhibited the growth of lung cancer cell line NCI-H446 cells, a dose-and time-dependent manner. For 24 h, half of the cells of the NCI-H446 cells 48 h after inhibition concentration (IC50) for 122.85,70.17 μg / mL, respectively. Volatile oil Anoectochilus can induce apoptosis in lung cancer cells NCI-H446 cells detected by TUNEL method, transmission electron microscopy method and observation, can be observed. DNA agarose gel electrophoresis DNA-ladder ladder bands. Conclusion: higher the volatile oil Anoectochilus ingredients; steam distillation of volatile oil extraction method suitable Anoectochilus, consuming short, the advantages of organic solvents with less; the volatile oil Anoectochilus ingredients can be inhibited in vitro cultured tumor cells growth and promote tumor cell apoptosis.

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