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Objective: In this study, through the application of cationic bovine serum albumin (C-BSA) induce Heymann rat model, benazepril hydrochloride as a control, and to explore the role of the safflower yellow the injection on heymann rats protected on the MMP -7, TIMP-1 impact of its experimental basis and theoretical support for the clinical treatment of idiopathic membranous nephropathy. Methods: 40 healthy male SD rats, adaptive feeding one week, urine protein tests negative, ten randomly selected as the normal control group, the remaining thirty SD rat tail vein injection of C-BSA copy Heymann rat model, each Week three times, four weeks after the injection, 24-hour urinary protein tests confirm successful modeling thirty heymann rats were randomly divided into model group, Tony enalapril treatment group, safflower yellow treatment group three groups Each group of ten rats, benazepril treatment group given benazepril hydrochloride 10mg? (kg)? d orally., safflower yellow pigment treatment group are safflower yellow injection 26.7 mg of / (kg · d) intraperitoneal injection, the normal group and model group fed with normal saline, the rats normal food and water, the continuous administration around. Four weeks after the detection intravenous injection of C-BSA and administered after four weeks of treatment, 24 h urinary protein excretion in rats. The end of the testing laboratory in the serum total protein (TP), albumin (ALB), total cholesterol (TC), triglyceride (TG), blood urea nitrogen (BUN), creatinine (Scr), followed by the rats were sacrificed -7 (MMP-7) mRNA in kidney specimens from kidney tissue, light microscopy, immunofluorescence, and electron microscopy pathological changes of renal tissue, in situ hybridization matrix metalloproteinase inhibitor -1 (TIMP-1), matrix metalloproteinase semi-quantitative analysis of tissues and pathological image analysis system. Results: 1,24 hour urinary protein determination after continuous intravenous injection of for C-BSA4 weeks, compared with the normal group, the model appears massive proteinuria, with a significant difference (P lt; 0.05); 4 weeks after administration compared with the model group, benazepril group and the treatment group safflower yellow 24-hour urinary protein excretion was significantly lower statistically significant (P lt; 0.05) between the two treatment groups showed no significant difference (P gt; 0.05). Biochemical indicators in each group after four weeks of continuous administration detection compared with the normal group, model group, benazepril group, safflower yellow pigment treatment serum TP, ALB significantly reduce TC, TG, were significantly increased, significant difference (P lt; 0.05); compared with the model group, benazepril group, safflower yellow treatment serum TP, ALB were significantly elevated TC, TG, were significantly lower, with a significant difference (P lt; 0.05); safflower yellow pigment treatment group compared with the benazepril treatment group difference is not obvious, no statistical significance (P gt; 0.05); serum BUN, Scr comparison, no statistically significant (P gt; 0.05); the renal pathological morphology observed under light microscope observation of normal fabric did not change significantly. Model group after Masson staining seen after glomerular volume corresponding increase, the glomerular basement membrane showed diffuse thickening of the tubular epithelial cells vacuolar degeneration and granular degeneration, renal interstitial lymphatic and monocyte cell infiltration, fibrosis. Safflower yellow pigment treatment group and benazepril rats glomerular volume only seen slight increases ball cells increase. Electron microscope observation of normal renal tissue memory in a very small amount of electron-dense material deposition, podocyte foot processes arranged in neat rows. Model rats showed the most electron dense deposits within the glomerular basement membrane, the large number of electron-dense material surrounding basement membrane hyperplasia, thin film matrix hyperplasia foot cell foot processes diffuse fusion. Safflower yellow pigment treated rats glomerular basement membrane memory in a small number of electron-dense material deposition, part of the podocyte foot processes began to diffuse fusion. Benazepril glomerular basement membrane electron-dense material with a certain deposition, electron-dense material around the thickening of the basement membrane hyperplasia, part of the podocyte foot processes showing diffuse fusion. The immunofluorescence normal group rats showed no significant performance model of renal tissue visible IgG and C3 was the fine granular along the glomerular capillary wall and part of the mesangial area showed varying degrees of deposition. The safflower yellow treatment renal tissue only visible visible mild IgG and C3 deposition, benazepril renal tissue presents a small amount of deposition of IgG and C3 was finely granular along the glomerular capillary wall . 4 renal tissue TIMP-1, MMP-7 mRNA in situ hybridization results TIMP-1 mRNA in tubular epithelial cells of normal renal tissue, a small amount of glomerular expression; model group and treatment group tubular epithelial cells and glomerular expression. The statistical analysis showed that, compared with the normal group, model group, glomerular TIMP-1 expression was significantly enhanced, a significant difference (P lt; 0.05), compared with the model group, the treatment group of TIMP-1 decreased statistically significant difference was significantly (P lt; 0.05), between the two treatment groups showed no significant difference (P gt; 0.05); small amount of expression of MMP-7 mRNA in the normal group tubular model group and treatment group were expressed in the kidney tissue, mainly expressed in the cytoplasm of renal tubular epithelial cells and glomerular, compared with the normal group, MMP-7 mRNA expression was significantly increased in the kidney of the model group (P lt; 0.05), with the model group compared to the two treatment groups increased expression (P lt; 0.05) between the two treatment groups showed no significant difference (P gt; 0.05). In MMP-7/TIMP-1 compared with the normal group, model group decreased significantly (P lt; 0.05), compared with the model group the the MMP-7/TIMP-1 recovery in the two treatment groups upregulation (P lt; 0.05 ) between the two treatment groups showed no significant difference (P gt; 0.05). Conclusion: safflower yellow injection can significantly reduce nephropathy rat urine protein and plasma proteins, improving lipid metabolism, protect renal function. 2 safflower yellow injection can regulate TIMP-1, MMP-7 mRNA expression in renal tissue, making MMP-7/TIMP-1 ratio increases, delaying glomerulosclerosis and to alleviate chronic renal injury, delaying membranous nephropathy further development. 3 safflower yellow injection has a significant therapeutic effect of membranous nephropathy in rats, the efficacy of benazepril hydrochloride is basically the same.
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