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With the improvement in the level of the accelerated pace of urbanization and industrialization, excessive domestic pollution emissions directly caused eutrophication, severe pollution, and even lead to black smelly water to form a black-odor water. Black-odor of the water is a very serious water pollution phenomenon, it completely lost the use of, and impact on the landscape, and human life and health of the water body. Black-odor water mostly in black-odor index and black-odor governance, and to solve the urban and rural water black-odor problem, we must first clarify water black-odor formation conditions. Range of water quality parameters of black-odor is not clear, therefore, by the homemade typical black-odor water, set the total carbon, total nitrogen, total iron concentration, as well as different temperature, dissolved oxygen, water depth of six impact factor analysis black-odor formation of water conditions. The results show that the black-odor of the water body forming conditions were as follows: total carbon (TC) ≥ 150 mg / L, total nitrogen (TN) ≥ 50 mg / L, total iron (TFe) ≥ 0.2 mg / L, temperature (T) ≥ 25 ℃, the depth (D) ≥ 0.9 m, CODcr ≥ 292 mg / L, C / N between the range of 3:1 to 4:1, as well as anaerobic conditions. Organic contaminants, inorganic contaminants, dissolved oxygen, temperature important factors affect black smelly water. To learn more about the combined effects of a variety of water quality and environmental factors affect black-odor formation of water, the paper design of orthogonal test. The study results showed that: the dissolved oxygen, the organic content of water bodies paddlewheel greatest impact, the optimal combination: A3 levels (200 mg / L), the B3 level (100 mg / L), the C2 level (anaerobic), D1 level (25 ℃). That is, when the concentration of organic matter in the water body TC 200mg / L, TN of 100 mg / L, T is 25 ° C, strictly anaerobic conditions in the water body most prone to black-odor. Black-odor during the formation of the body of water, microbes play a huge role. Analysis of black-odor water microbial community structure differences, understanding the different locations and the same location at different depths of black-odor water bacterial diversity is directly extracted from Wuhan Nanhu outfall, black-odor river waters Patrol Division microbial DNA and their communities by DGGE fingerprinting analysis. The study results showed: (1) black-odor river waters of South Lake outfall and Patrol Division about 12 kinds of dominant flora, and the same location (South Lake outfall) different depths DGGE profiles were not significant, the bacterial community structure have higher similarity, DGGE strip with different Location (Patrol Division River), the number and location of a significant difference. (2) addition to the distribution of approximately three of the same bacterial populations, the two sample points, and also having a specific microbial species may be related to the physicochemical properties of the water body.
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