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The Cerasus cherry (Prunus campanulata Maxim) chilling requirement at least in the world, the most gaudy colors of cherry varieties, but poorly understood, in addition to Japan, cherry blossoms and the campanulata crossbreeding research has not yet been in Taiwan The campanulata source of the original species and varieties of improved varieties. In this study, the use of introduction from Taiwan Fujian mountain cherry original species and varieties with cold cherry cherry Alishan, King Weng cherry and thin ink cherry conduct cross-breeding, and using SRAP marker analysis between parent and offspring kinship relations, results are as follows: 1 16 Shape of parental performance and stability can be used for identification of the species classification traits is whether the inflorescence indumentum, Corolla hair, sepal number and shape after flowering, anti-roll or not, the shape of the calyx tube, petals flap 10 traits, petal apex shape, inflorescence, fruit color traits. 2.8 parental color distribution table on the color system coordinate system in the CIE L * a * b *. 8 parental suit the lightness of the colors of the L * value was measured with a colorimeter, a hue a *, b * values, the calculated chroma C * and hue angle H / °. Eight parental lightness L * value with red, green and attributes a * value, chroma value C * values ??were negatively correlated most strongly, the 5th parental suit with red accents, blue tones strongest, minimum brightness, saturation value; No. 6 red tones weakest parental suit, blue tones weakest, lightness, chroma values ??minimum consistent with the naked eye observation. 3.8 pro of floral organ analysis of 11 traits. 11 characters length and width measurements with a vernier caliper measurements, No. 4 pro the bract length and width, peduncle maximum and pedicels value are the spendthrift the 5th parental longest, the longest calyx is the 8th parental petals longest is the No. 6 and No. 7, the parental, No. 3 parental petals widest longest No. 1, the parent of stamens and pistils longest No. 5 parental. 4 main factors affect the campanulata its closely related species of floral character. The principal component analysis campanulata and its related species morphological classification of the main factors, the results show that the the spendthrift length, total stem length, petal length and width, bract width factors campanulata and its related species of floral organ morphological differentiation The main factors. 27 combinations of hybrid offspring seed germination and planting seedlings survival rate of the statistics, most self-crosses the 8th pro this to get the seed number, the same germination time on the 8th pro this the germination effect than in the 4th pro this good, the 4th pro this self-cross seed seedling survival rate is high; hybrid combinations in the 2nd parent as the male parent, the 4th parents as a higher rate of female parent seed harvest, better germination, the highest survival rate of hybrid seeds. 6.8 parental floral character clustering. Eight parents can obviously be divided into four groups: 3, No. 4 and No. 2 parents gathered in a major category, the 1st and the 5th parents gathered in a large class, No. 7 and No. 8, parents gathered in a large class 6 parents alone together into a class, the farthest distance and other major groups. 7 campanulata and its closely related species SRAP-PCR reaction system, i.e., 25ul of the reaction system, dNTP concentration for 0.12mmol / l, the concentration of Taq enzyme 1.00U with Mg2 concentration of 1.50 mmol / l Preparation primer concentrations 0.30umol / l, and the remaining double-distilled water topping. The amplification reaction program was 94 ° C denaturation 5min; denaturation at 94 ° C for 1 min, 35 ° C annealing 1min, extension at 72 ° C for 1 min, 5 cycles; denaturation at 94 ° C for 1 min, 50 ° C annealing 1min, extension at 72 ° C for 1 min, 35 cycles ; 72 ℃ for 10min; stored at 4 ° C or -20 ℃. 8.13 primers detected a total of 111 alleles were detected number of each site ranged from 6 to 12. The average number of observed alleles per locus of 8.5 polymorphic rate of 84.6%. 9. The eight parents SRAP data clustering. Eight parents can be divided into 3 groups: No. 4, No. 5, No. 3, 2 and 1 Hao pro this poly into a large class of group No. 6 parents alone for a taxa, No. 7 and 8 Hao pro this poly into a taxa. 10 parent and offspring the SRAP genetic distance clustering. Clustering of 34 individuals for the independent parent of No. 1 and No. 6 pro-groups 2 parental hybrids 24 and 23 for group; No. 3 and its hybrids 342,351,352,5 parents selfing 551 varieties and 552,5 parent so close genetic distance clustered into groups; 4 parents and their hybrids 341,411 the selfing species 441, 442, 443, etc. groups; parents in the 7th the selfing species genetic distances of 772 and 775 over 771 and 777,773 and 774 near the former first clustered into a small group, the latter separately clustered in a small group, three sub-categories with the 7th parental self species 776 poly for a large class; No. 8 parents and their cross-species 883 first a subset of a subset of 881 and 882,885 and 886 due to the short distance, but also independent poly, then self-cross hybrids 884 and 887 together again for a large class.
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