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In this study, paired the straw mushroom single-spore experimental strains of the straw mushroom total RNA extraction methods, and the use of high-throughput sequencing technology of digital gene expression profiles on different developmental stages of the straw mushroom stipe tags sequencing grass during the development of mushroom button of the stipe (Nb), the egg stage Stipe (Db), elongation Stipe (Sb), expression profiling of mature stipe (Cb) four periods. Bioinformatics expression profiling analysis of the four periods. By high-throughput sequencing, we obtained various sample library 5.7M-6.2M label, library label number of species distributed between 0.1-0.3M. Remove the low quality of the pins, the total number of the Clean tag Nb, Db, Sb, Cb four periods respectively 5612361,5978522,5972497,5967968; 90437,97022,87911,83702 number of pins species obtained. Sequencing label with the reference sequence for comparison, Nb, Db, Sb, Cb four library than to the number of reference transcripts were 10610,15329,13815,14335. Four samples pins copy number changes showed the same trend. Sequencing pins and the antisense strand also has a partial match, Nb, Db, Sb, Cb four libraries were about 34.40%, 35.83%, 31.83%, 31.85% of the reference genes specific pins reverse match, these genes there may be a bi-regulated genes. Detected in Nb, Db, Sb, Cb four times with the growth and development of related proteins, chitin synthase, and mitogen protein kinase, endoglucanase, actin, tubulin, drive protein expression levels in different developmental stages straw mushroom is different, but similar trends, these proteins for the in Db period corresponding transcript content is higher, suggesting that Db period of strong growth, the energy needed more substance. Cell wall synthesis of transcript levels at a very high level, the tubulin of transcript levels are high, in the the Db period and Sb period speculated that the transcription of the, Sb period stipe elongation related. GO functional classification of differential gene significantly enriched GO entry analysis of transcripts related to bind to DNA, chromosomes, transferase activity, ribosomal protein related. By analyzing the speculation the Db period compared with Nb period strong DNA replication, rapid cell division and differentiation, there is more regulation of signaling molecules; Db and Sb period compared to a large number of DB period ribosomal speculate DB period protein the synthesis of many types and quantities of straw mushroom stipe into the elongation has been completed differentiation protein synthesis ability and Sb compared to the period weakened; Sb period compared with the period of Cb, Cb period histone synthesis is blocked, presumably Cb period of grass the mushroom chromosome activity is low, reduced ability to replicate, there is the phenomenon of aging, does not need more energy supply. Significantly enriched in the metabolic pathway analysis, DNA replication, Ribosome, Cell Cycle and other metabolic pathways, was found by analyzing the DNA replication pathway Nb, Db, Sb, Cb four periods during DNA replication capacity Db strong, with the development gradually weakened; in Risome pathway, Nb, Db, Sb, Cb four periods, Nb and Db period ribosome compared with the SB, Cb period a large number of; Cell Cycle pathway DB during the cell cycle activities more . Comprehensive analysis suggesting that in straw mushroom during the development of Nb to DB period of strong growth, the ability of life, cell proliferation and differentiation are more active, there is more signal-regulated activities stipe elongation at this stage is mainly dependent on cell division and proliferation , Db to Cb period Stipe, elongation to rely more on cell elongation. Db period of strong ability of DNA replication, protein synthesis at its peak, the metabolism of the period, with higher levels of secondary metabolites, the straw mushroom periods of high nutritional value. Comparison of the four libraries, we found that the number of unique transcripts in Nb, Db, Sb, Cb four times 22,264,154,129 to maintain a specific period of time, suggesting that these unique transcripts and straw mushroom biology characteristics.
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