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The research background psoriasis (Psoriasis, OMIM * 177900) is a long-term chronic inflammatory skin disease, the genetic and environmental factors in the development of the disease process plays an important role, according to statistics the incidence of the Han people is 0.123%. Psoriasis vulgaris (Psoriasis vulgaris, PV) is the most common clinical type, accounting for more than 99%. Our group Psoriasis genome-wide association analysis (Genome-wide association study, GWAS) study found that the Han people with psoriasis vulgaris susceptibility SERPINB8 (rs514315) gene polymorphisms significantly associated. Objective analysis stratified by the psoriasis vulgaris age of onset, family history and clinical type SERPINB8 (rs514315) polymorphisms with psoriasis vulgaris patients with clinical phenotype correlation study in the Han population, to further explore the silver the crumbs disease pathogenesis provides an important basis of genetics. Methods 7,227 patients with psoriasis and 11,313 cases the control SERPINB8 gene polymorphic loci genotyped rs514315 (CC, CT, TT) information part comes from our group pre psoriasis GWAS data (including 1,031 cases of silver Illumina 610 chip crumbs patients and 1,120 normal controls) genotyping results, the other part comes from validation (4,338 psoriasis patients and 5,058 normal controls) and validation 2 (1,858 psoriasis patients and 5,135 patients the the control) the Sequenom MassArray system and Biosystems TaqMan assays genotyping genotyping data. By appropriate transformation of the data after the application of social science statistical package SPSS 10.0 for data for statistical analysis. χ 2 tests were used to compare groups rs514315 genotype SERPINB8 gene polymorphic loci and allele frequency distribution. Results 1. Distribution of the genotype frequencies of the case group and control group overall difference was statistically significant (χ 2 = 30.22, df = 2, P = of 2.74 × -7 case group and the control group, the allele frequency distribution differences statistically significant (χ 2 = 30.15, df = 1, P = of 3.99 × -8 OR 1.15,95% CI 1.09-1.20). 2. Children onset patients compared with the control the rs514315 locus genotype and allele distribution of the differences were statistically significant (χ 2 = 19.23, df = 2, P = 6.67 × 10 -5 ; χ 2 = 19.08, df = 1, P = 1.25 × 10 -5 , OR 1.21,95% CI 1.11-1.31). patients with adult-onset compared with the control rs514315 genotype and allele distribution differences with statistical significance (χ 2 = 19.54, df = 2 , P = 5.72 × 10 -5 ; χ 2 = 19.46, df = 1, P = 1.02 × 10 -5 , OR 1.13, 95% CI 1.07-1.19). Children onset patients and patients with adult-onset between rs514315 genotype and allele distribution differences were not statistically significant (the χ 2 = 2.18, df = 2, P = 0.34; χ 2 = 2.10, df = 1, P = 0.15, OR 0.94,95% CI 0.86-1.02). 3. patients with a positive family history compared with the control, rs514315 genotype and allele distribution differences were statistically significant (χ 2 = 17.96, df = 2, P = 1.26 × 10-4; χ 2 = 17.08, df = 1 , P = 3.59 × 10 -5 , OR 1.19,95% CI 1.10-1.30). patients with a family history of negative comparisons, rs514315 genotype and allele distribution difference was statistically significance (χ 2 = 20.82, df = 2, P = 3.02 × 10 -5 ; χ 2 = 20.54, df = 1, P = 5.84 × 10 -6 , OR 1.13,95% CI 1.07-1.19). patients with a positive family history and negative patients between rs514315 genotype and allele distribution of the differences were not statistically significant ( χ 2 = 2.45, df = 2, P = 0.29; χ 2 = 1.31, df = 1, P = 0.25, OR 0.95,95% CI 0.87-1.04) 4 patients with chronic plaque compared with the control, the rs514315 locus genotype and allele frequency distribution of the overall differences were statistically significant (the χ 2 = 29.62, df = 2, P = 3.69 × 10 -7 ; χ 2 = 29.55, df = 1, P = 5.43 × 10 -8 , OR 1.16,95% CI 1.10 -1.23) the rs514315 locus genotype and allele frequencies between patients and control of acute guttate distribution overall differences were statistically significant (χ 2 = 6.02, df = 2, P = 0.049 ; χ 2 = 6.00, df = 1, P = 1.4 × 10 -2 , OR 1.10,95% CI 1.02-1.20). patients with chronic plaque and acute between patients with guttate the rs514315 locus genotype and allele frequency distribution differences were not statistically significant (χ 2 = 1.31, df = 2, P = 0.52; χ 2 = 1.31, df = 1, P = 0.25, OR 1.05,95% CI 0.97-1.15). associated gene (rs514315) conclusions 1. SERPINB8 of genetic polymorphism and the susceptibility of the Han population psoriasis vulgaris; genetic polymorphism and the age of onset of psoriasis 2.SERPINB8 gene (rs514315), with or without a family history, no significant correlation of the clinical onset of type.
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