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Housefly (Musca domestica) is an extremely wide range of important public health pest worldwide distribution, with the widespread use of chemical pesticides, more and more of the housefly populations resistant. Housefly is the focus of our biological vector control, the survey showed that many Chinese provinces housefly has a certain degree of resistance to different insecticides, which has become a major obstacle to the control of diseases spread by houseflies. Has become the focus of attention due to organophosphate (OP) and pyrethroid pesticide resistance problems of the mammalian low toxicity and housefly quickly downed characteristics can be widely applied, resulting in the housefly. Therefore, to carry out the housefly resistance monitoring for guiding rational use of pesticides and housefly comprehensive management of great significance. This article by bioassay housefly kdr resistance characteristics from the gene level, on the basis of proven throughout China housefly organophosphorus and pyrethroid insecticide resistance status quo, the use of molecular biology techniques , to provide a theoretical basis for further reveal the housefly the knockdown resistance mechanisms (kdr) as well as the establishment and improvement of the housefly resistance to molecular detection methods designed for the rapid detection of pest resistance, molecular detection method to extend the pesticide life. The results are as follows: 1.2010 measured in Shanghai, Beijing, Guangdong, Jilin, Shandong, Tianjin and other provinces housefly malathion, diazinon, permethrin, deltamethrin sensitivity. The results show that the housefly resistance to malathion has reached a high level. Highest resistance for the Beijing Tiantongyuan strains (TTY), LD50 value of 280.81 (219.85-377.51) μg / head, the resistance ratio of 305 times. All strains of diazinon were not significant resistance to permethrin and deltamethrin ZYH and strains cross-resistance exists. Malathion, the the four insecticides diazinon, permethrin, deltamethrin ZYH strains screened, get ZYH-M, the ZYH and-Di ZYH-P and ZYH and-De 4 different strains. After the multi-generational screening, except ZYH-Di strains, the other three strains resistant significantly increased. Allele-specific amplification (AS-PCR) amplified 500bp long fragment and to detect the F0 generation ZYH strain CYP6D1V1 genotype with restriction enzyme digestion, sensitive pure aggregate (SS), heterozygous (RS) and the resistant homozygous genotypes RR 3, its frequency was 90.43%, 7.45% and 2.18%, respectively. 4 design specific primers and establish the allele-specific amplification method (AS-PCR method), successfully detect the presence of 6 genotypes ZYH strains, respectively, the L / L, L / F, F / F, L / H, H / H, F / H genotype.
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