Dissertation > Excellent graduate degree dissertation topics show

Human dental pulp cells into odontoblast differentiation inducing direction of miRNA screening

Author: YangMingCong
Tutor: XiangXueRong
School: Chongqing Medical University
Course: Clinical Stomatology
Keywords: Human dental pulp cells miRNA Odontoblast Differentiation
CLC: R781.3
Type: Master's thesis
Year: 2011
Downloads: 75
Quote: 0
Read: Download Dissertation

Abstract


Background microRNAs (miRNAs) are small non-coding RNA, a length of about 20 ~ 22bp, transcribed from the genome, and the target gene mRNA through base pairing to guide silencing complex (RISC) mRNA degradation or inhibition of mRNA translation, resulting in transcriptional level regulating protein expression. As endogenous RNA, as both can fine-tune the molecular gene expression, which may be involved in organism growth, development, aging, and death regulation in recent years, in cell proliferation and differentiation in the sense caused wide concern. miRNA were expressed in a variety of cells, which negatively regulates cell through certain key genes for cell differentiation from regulation [1.2.3]. In the dental pulp tissue nutrient metabolism, sensory, repair, and other physiological functions play an important role in-depth study of pulp, helps us understand the differentiation of pulp cells, pulp calcification, dentin and related regulatory factors . Pulp cells can be induced to differentiate into odontoblast differentiation and the formation of dentin-like structure, indicating its presence in the dentin regeneration as seed cells application prospects. Objective To detect dental pulp cells and induce differentiation of odontoblasts of miRNA gene expression profiles, screening cells before and after induction of differentiation differences miRNAs, and predict their likely target genes, looking for possible human dental pulp cells into nature of differentiation-specific miRNAs, and validated, in order to further explore the biological function of miRNAs on HDPCs provide an experimental basis for regulation. Materials and methods (1) with enzyme digestion tissue cultured human dental pulp cells were isolated by observing the primary and passaged HDPCs morphological characteristics, immunochemical detection of cell surface markers on human dental pulp cells were identified; (2) using dexamethasone, b glycerophosphate and vitamin C combined to induce HDPCs odontoblast differentiation, alkaline phosphatase activity was detected; (3) the use of miRNA microarray technology to detect human dental pulp cells and induce differentiation The odontoblast expression profiling of miRNAs, analyzed and screened at HDPCs differentially expressed during the differentiation of miRNA, and through miRGen database to predict the target gene; (4) using semi-quantitative RT-PCR method, the miRNA microarray screening out its induced differentiation of human DPCs odontoblasts differences miRNA validated. Results (1) by enzymatic digestion method combined with cultured tissue derived cells, cell phenotype identified by immunohistochemistry: vimentin, I collagen expression was positive. (2) pulp cells containing dexamethasone,? - Glycerophosphate and vitamin C in the conditioned medium can be induced to differentiate into odontoblasts form calcified nodules, elevated alkaline phosphatase activity was detected, calcification Results Section Von Kossa staining positive. (3) the use of cDNA microarray analysis of human DPCs and induced differentiation of odontoblasts miRNAs expression profiles screened differentially expressed miRNAs, mineralization induced HDPC compared the expression profiles of non-induced HDPC comparison, there are 72 kinds of miRNA occurs upregulated more than 1.5 times, 61 kinds of miRNA downregulation occurred more than 1.5 times. (4) for the screening of differentially expressed miRNAs through miRNA target prediction tool analysis, with the target gene miRNA has 35, 35 of miRNA target genes sum of 1327, miRNA and target gene relationships to a total of 2158. (5) Select hsa-miR-150 by semi-quantitative RT-PCR to verify the results were consistent with the chip. Conclusions (1) with a tissue enzyme digestion can be isolated from human dental pulp of human dental pulp cells in vitro can be induced to differentiate into odontoblasts. (2) in human dental pulp cells and induce differentiation of odontoblasts are present in a large number of miRNAs. (3) in human dental pulp cells and induce differentiation of odontoblasts there are differences in the expression of miRNAs, they may odontoblast differentiation of dental pulp cells plays an important role in regulating the presence of so-called specific miRNA.

Related Dissertations

  1. Research on Disease Related miRNA Mining Based on Protein-Protein Interaction Network,R341
  2. Exoression of Resistin During Porcine Preadipocyte Differentiation and Its Infiuencing Factors,R587.1
  3. miRNA-23a into the regulation of muscle differentiation in C2C12 cells,Q952.5
  4. Evaluation of Land Use Eco-security in Fujian Province,X826
  5. Variations of Host-Specialized and Migratory Biotypes of Cotton Aphid on the Mating Behavior and Genes Sequences of Mitochondrion DNA and Symbiotic Bacteria,S433
  6. The Molecular Systematics and Classification of Triticeae Polyploids with St Genome,S512.1
  7. Effects of Isoprocarb Exposure on Differentiation, Migration and Neurite Outgrowth of the Key Cell During Neural Development in Vitro,R329
  8. The Study of Rabbit Bone Marrow Mesenchymal Stem Cells Separation Culture and Directional Differentiation,R329
  9. Identification of Biological Characteristics with Human Keloid Fibroblast-like Mesenchymal Stem Cells,R622
  10. Study on the Physiological Differentiation and Genetic Diversity of Exserohilum Turcicum in Northeastern China,S435.131
  11. Optimization Study on BHF Marketing Strategy,F274
  12. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  13. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  14. The Mechanism of CREG Regulated Vasculogenesis in Murine Embryonic Stem Cells,R541.4
  15. Research of Differentiation of Human Peripheral Blood Mononuclear Cells into Hepatocyte-like Cells in Vitro,R575.3
  16. Guangzhou Daily Newspaper Group, the industry value chain research,G219.24
  17. Based Capital Perspective outdoor new media strategic research and case studies,G206-F
  18. Eastern China in the New Comparative Study of differentiation of the peasantry,D663.2
  19. A Research on the Polyphones in Shuowenjiezizhu,H161
  20. Construction of Silence Vector for Cytochrome b Gene in Nucleus of Arabidopsis Thaliana and the Transformation,Q943
  21. MicroRNA Expression Profile and Computational Analysis of Gene Expression in Human Endometrium During the Implantation Window.,R321

CLC: > Medicine, health > Oral Sciences > Oral medicine > Endodontics and periapical disease
© 2012 www.DissertationTopic.Net  Mobile