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Objective To investigate the the tiny RNA precursor (pre-miRNA) region gene polymorphism with susceptibility to the Chinese population hepatocellular carcinoma (hepatocellular carcinoma, HCC) relationship . Methods A case - control study design, including the diagnosis of HCC patients , 963 cases of hepatitis B virus (hepatitis B virus, HBV) positive control of 829 cases and 852 HBV negative control . Select the pre-miRNA region polymorphic loci hsa-mir-146a rs2910164 C → G of hsa- mir - 196- a2 rs11614913 T → C for the study sites , the application of primer mismatch restriction analysis ( primer introduced restriction analysis- PCR PIRA-PCR) method polymorphism detection using logistic regression calculated OR and 95% CI, comparing different genotypes and HCC risk of relationship . The rs2910164 site 3 genotypes CC, CG and GG in the frequency distribution of the patient group were 34.5% ( 319 / 925 ) , 48.6% ( 450 / 925 ) and 16.9 ( 156/ 925 ) in HBV - positive control group , respectively 36.4% ( 274 / 753 ) , 45.0% ( 339 / 753 ) and 18.6% ( 140/ 753) , in HBV negative control group were 36.1% ( 303 / 840 ) , 46.0% ( 386 / 840 ) and 18.0 % ( 151 / 840 ) . rs11614913 point 3 genotypes TT , CT and CC distribution frequency in the case group were 29.7% ( 277 / 934 ), 48.1% ( 449 / 934 ) and 22.3% ( 208 / 934 ) in HBV - positive control group , respectively 30.3% ( 238 / 785 ) , 51.0% ( 400 / 785 ) and 18.7% ( 147 / 785 ) , 28.6% ( 239 / 837 ) , respectively, in the in HBV negative control group , 49.8% ( 417 / 837 ) and 21.6 % ( 181 / 837 ) . Adjusted for age, gender , smoking , alcohol drinking , failed to find the two polymorphic and between HCC incidence dangerous in statistically significantly associated ( compared with the positive control of HBV : hsa - mir - 146a rs2910164 : correction OR = 1.10 95% CI = 0.90-1.36 (GC / GG vs CC) ; hsa - mir -196 - a2 rs11614913 : the correction OR = 1.01, 95 % CI = 0.81-1.25 (CC / CT vs TT ) ; HBV negative photographic than : hsa-mir-146a rs2910164: the correction OR = 1.06, 95% CI = 0.87-1.29 ( the GC / GG vs CC) ; hsa - mir - 196- a2 rs11614913 : correction OR = 0.94,95% CI = 0.76-1.16 (CC / CT vs TT)). Analysis stratified by age, sex , smoking, alcohol consumption , also failed to find the two polymorphic loci danger associated with HCC incidence . Conclusion hsa - mir- 146a rs2910164 C → G as well as the hsa- mir -196 - a2 rs11614913 T → C polymorphism may not Chinese crowd HCC susceptibility markers .
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