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Objective: To detect matrine HepG2 cells , NF -κB ( p65/RelA ) of p53, decoy receptor 3 (decoy receptor3, DcR3/TR6) mRNA expression levels to explore matrine on HepG2 cells may the mechanism of action, as well as the interaction between these cytokines . METHODS: Cultured hepatoma cell line HepG2 . Matrine concentration gradient stimulate hepatoma cells (0g / L , 0.25g / L , 0.5g / L , 1.0g / L , 1.5g / L) cultured hepatoma cell lines are divided into five groups , LY294002 ( broad-spectrum inhibitors of PI3K/Akt signaling pathway ) stimulate the liver cancer cells as a positive control group , and the other set a negative control group . MTT assay were used to detect the six cell culture inhibition rate of 12, 24, 36, 48 hours . Apoptosis morphological changes after 12 hours of incubation with Hoechst staining . The real-time fluorescence quantitative polymerase chain reaction (real-time quantitative polymerase chain reaction, RT-qPCR) to detect cells of NF-κB, p53, DcR3 mRNA expression levels . RESULTS: Matrine induced apoptosis of hepatoma cells in a time- and dose-dependent . The matrine final concentration of 1.5g / L culture medium 12 hours to cause obvious apoptosis of hepatoma cells . NF-κB mRNA expression with increasing matrine concentration increased ( P = 0.017 ) , p53 mRNA expression was lower ( P = 0.007 ) with the increase in the concentration of matrine , NF -κB p53 mRNA expression was negatively related DcR3 mRNA expression from matrine concentration . LY294002 treatment of liver cancer cells , NF -κB , p53, of DcR3 mRNA expression compared with the control group was significantly higher (P lt; 0.001). Conclusion: Matrine induced apoptosis of hepatoma cells activation of NF-κB, which may be one of the mechanisms of liver cancer cells on the of matrine early resistance . Matrine may be affected by the effects of the cell types of the role of NF-κB .
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