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Objective: To observe the bone morphogenetic protein -2 (BMP-2), thermo-chemotherapy ( five fluorouracil , 5 - FU ) alone or combined with human colon cancer cells (SW480) strains and to study its possible mechanism of proliferation and apoptosis . : BMP-2 (25,50,100,200,400 μg / L) role in SW480 cells , MTT assay detected cell viability . BMP-2 (100μg / L) ,5-FU (50mg / L) separate and combined effects of the SW480 cell line , FCM assay apoptosis rate , Western blot, detection of PTEN phosphatase ( human chromosome 10 deletion tensin homologue gene ) expression of the protein , reverse transcriptase polymerase chain reflect (RT-PCR) to detect the expression of PTEN mRNA . Results : BMP-2 can inhibit the proliferation of SW480 cells . Comparison with the separate role , the role of BMP -2 joint thermo-chemotherapy (5-FU) in SW480 cells , apoptosis rate was significantly higher (P lt; 0.05). BMP-2 can increase the level of expression of the PTEN protein . The groups between PTENmRNA expression difference. Conclusion : The combined effects of BMP -2 and thermo-chemotherapy (5-FU) synergistically induced apoptosis of SW480 cell lines , and can improve the thermal sensitivity of chemotherapy , which may be related to the increase in PTEN protein expression .
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