Dissertation > Excellent graduate degree dissertation topics show

Effect of Methylene Blue-Mediated Sonodynamic Action on Apoptosis and Metastasis of Ovarian Cancer Cells

Author: XiangJunYan
Tutor: XuChuanShan
School: Chongqing Medical University
Course: Medical Imaging and Nuclear Medicine
Keywords: sonodynamic therapy ovarian cancer MB apoptosis
CLC: R737.31
Type: Master's thesis
Year: 2011
Downloads: 12
Quote: 0
Read: Download Dissertation

Abstract


Background and objective: Ovarian cancer is the leading cause of death in gynecological cancer. Chemotherapy plays an important role in treatment of ovarian cancer, however, the long-term efficacy is still unsatisfactory. With the development of ultrasonics, sonodynamic therapy has become a new modality for treating tumor because of its advantages in tissue penetration in comparison with photodynamic therapy. Sonosensitizer is a key component affecting SDT’s efficacy. Methylene blue(MB) is a second-generation photosensitizer from phenothiazine dye. Recen study showed that MB might be a sensitizer to enhance ultrasound therapy on tumor. Our aims are to explore the mechanism of MB-induced sonodynamic therapy on ovarian cancer cells.Materials/Methods: Cytotoxicity was detected using trypan blue exclusion test after treatment with MB-mediated SDT. Light microscope was used to observe the morphological changes of the HO-8910 cells. Apoptosis was evaluated using FCM with Annexin V-FITC/PI staining, and the nuclear staining with Hoechst 33258. Ultrastructure was observed by TEM. Mitochondrial membrane potential was evaluated using CLSM with rhodamine123 staining. Intracellular reaction oxygen species were detected by DCFH-DA staining. Finally, clone assay, invasion assay, migration assay, and adhesion assay were investigated in this study.Results: The cytotoxicity of MB-mediated SDT on HO-8910 cells after MB-mediated SDT was significantly higher than those of other treatments including ultrasound alone, MB alone and sham treatment. And the above finding was also confirmed by inverted light microscopy. Nuclear staining showed DNA condensation and fragmentation after MB-mediated SDT. FCM showed the MB-mediated SDT significantly increased the early or late apoptotic rate up to 7.91% and 18.17% compared to the controls. The apoptotic bodies of the HO-8910 cells can be found by TEM after MB-mediated SDT. The collapse of mitochondrial membrane potential and ROS increase were found in HO-8910 cells after MB-mediated SDT. And adhesive, migrative and invasive ability of HO-8910 cells were significant inhibited after MB-mediated SDT.Conclusion: MB-mediated SDT could significantly kill ovarian cancer cells and inhibit the spread of the cells. Our results indicated that MB was a novel sonosensitizer and MB-mediated SDT might be a new therapeutic modality in the treatment of ovarian cancer.

Related Dissertations

  1. Video Coding Research Based on Texture Characteristics,TP391.41
  2. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  3. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  4. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  5. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  6. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  7. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  8. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  9. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  10. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  11. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  12. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  13. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  14. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  15. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  16. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  17. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  18. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  19. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  20. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  21. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3

CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
© 2012 www.DissertationTopic.Net  Mobile