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Studies on the Mechanism of Endoplasmic Reticulum-mediated Apoptosis Induced by Dihydroartemisinin in PC-3 Cell Lines of Prostate Cancer

Author: LiJian
Tutor: LuoZiGuo
School: Chongqing Medical University
Course: Human Anatomy and Embryology
Keywords: Dihydroartemisinin Prostate cancer cells-3 Apoptosis Endoplasmic reticulum stress CHOP/GADD153
CLC: R737.25
Type: Master's thesis
Year: 2011
Downloads: 51
Quote: 0
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Abstract


Objective: To investigate the mechanism of endoplasmic reticulum-mediated apoptosis induced by DHA in PC-3 cell lines of prostate cancer.Methods: PC-3 cells were treated with DHA. The rate of apoptosis and intracellular calcium concentration of PC-3 cell were detected by flow cytomery analysis. Reverse transcription-polymerase chain reation（RT-PCR） was used to measure the expression of mRNA about CHOP、ATF4、ATF6、XBP1、Bcl-2 and Bax. Immunocytochemistry was used to deteched the expression of CHOP protein.Results: The rate of apoptosis increased after DHA treatment in a time and concentration-dependent manne. There was no significant difference in intracellulai calcium concentration of control and solvent group（P>0.05）. After 36h treatment of DHA 100μmol·L-1,the intracellulai calcium concentration increased but no significant defference（P>0.05）. I t increased markedly after 48h treatment of DHA（P<0.05）. After 8h treatment of DHA 200μmol·L-1, the intracellulai calcium concentration increased（P>0.05）. It increased markedly after 24h and decreased after 36h peak concentration（P<0.05）. The expression of CHOP、ATF4、ATF6、XBP1 and Bax mRNA were up-regulated and Bcl-2 mRNA was down-regulated after DHA treatment by RT-PCR analysis. The immunocytochemistry showed up-regulation of protein of CHOP.Conclusion: DHA could activate the three signal passageways of UPR （PERK、IRE1 and ATF6）,and induce apoptosis of PC-3 cells. CHOP play an important role in this process. The endoplasmic reticulum stress-mediated apoptotic pathway is involved in this action of DHA. Calcium、Bcl-2 and Bax may be ligaments between ER- and other pathways,but the mechanism is not be confirmed further.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Male genitalia tumors > Prostate cancer
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