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Experimental Study of the Effects of Rh-endostatin on the Metastasis of VX-2 Popliteal Lymph Node of Rabbits

Author: TanYuJun
Tutor: YuZhengYang
School: Nanhua University
Course: Surgical Oncology
Keywords: Rh-Endostatin Angiogenesis Lymphangiogenesis Lymphaticmetastasis VX-2
CLC: R738.7
Type: Master's thesis
Year: 2011
Downloads: 27
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Abstract


Purpose:(1) Observing rh-endostatin to inhibit Angiogenesis and Lymphangiogenesis of the implanted VX-2 tutor, and discussing the functions of the implanted VX-2 tutor building and the metastasis of popliteal lymph nodes.(2) Through counting the microvessel density (MVD) of the popliteal lymph nodes issue of implanted VX-2 tumor to rabbits and Lymph nodes matrix ,and observing the shape,distribution characteristics of the microvessel in the popliteal lymph nodesMethods:Selected 12 New Zealand white rabbits for the laboratory of the University of Nanhua, and implanted VX-2 rabbit tumor issues(1mm~3)in the muscles of their right hind legs to build the model of implanted VX-2 tutor, and the implanted tumors grown to 3mm in diameter after a week. We divided them randomly into two groups, for the experimental group, we kept using 0.75mg/Kg Endostar intravenous injections in ears vein for two weeks to inhibit the growth of VX-2 implanted tumors, and we used physiological saline intravenous injections for the matched group which also lasted for two weeks. And used color Doppler ultrasound once a week to measure the volume change of the tumors and the change of blood supply within the tumors , then we drew a curve to illustrate the growth of the tumors and calculate the inhibition rate(IR). After using Endostar for a week, we took out the tumor tissues and popliteal lymph nodes of the two group, with the help of immunohistochemistry technology, we marked microvessels and microlymphatic vessels in tumor tissues by CD31 and LYVE-1 respectively and recorded the MVD and MLVD for the two group, and marked microvessels in popliteal lymph node tissues by CD31 for the two group and recorded its MVD, and checked the VEGF in the tumor tissues of the two group.Adopt an injection with a dose of ABS(red) the lymph nodes introduction of the arterial blood for blood to the nodes with type,indirectly at the same time the lymph application of technology to inject injected with a dose of ABS(blue)observed all nodes. When completed shall form a unit of type, trim, the flushing, slice, dry and conduct electricity,etc. the scanning and camera is focused on laser of reconstruction. the three-dimensionalResults:(1)Endostar has performed well in inhibiting the metastasis of the popliteal lymph nodes in VX-2 muscles implanted tumors for rabbit(sP<0.05);it has also a remarkable performance in inhibiting the tumor growth and the blood supply inside (all the Ps are below 0.05), and it has achieved IR from 26.22% to 29.09%.(2)The MVDs in VX-2 rabbit tumor tissues and popliteal lymph node tissues of the matched group are higher than those of the experimental group (P<0.05).(3)In the matched group, the MLVDs of VX-2 rabbit tumor tissues are higher than those of the experimental group(P<0.05).(4)The VEGF positive rates of cell percentage in the VX-2 rabbit tumor issues of the matched group(30.10±3.89)are higher than those rates of the experimental group(20.59±3.59), which has a statistical significance(P<0.05).(5)The VEGF rate of the VX-2 rabbit tumor cell in the matched group correlates positively with the MVDs in the VX-2 rabbit tumor issues and popliteal lymph node tissue(sr=0.89,P =0.000;r=0.79,P =0.001), as well as the MLVDs of the VX-2 tumor tissues,(r=0.87,P =0.001). The VEGF rate of the VX-2 rabbit tumor cell in the experimental group correlates positively with the MVDs in the VX-2 rabbit tumor issues and popliteal lymph node tissues(r=0.85,P =0.003;r=0.81,P =0.006), as well as the MLVDs of the VX-2 tumor tissues,(r=0.82,P =0.001).Conclusion:(1) Endostar can inhibit the VEGF positive high rate of VX-2 rabbit implanted tumors, And influence the rate of MVD and MLVD of VX-2 rabbit implanted tumors.(2) Endostar can inhibit the metastasis of its popliteal lymph node,and influence the rate of MVD of the metastatic lymph node.

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