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Objective To study the Bai Yang Suyan bio 8 - bromo-7 - methoxy chrysin (BrMC) suppression vitro cultured human lung adenocarcinoma A549 cell growth and induced apoptosis of A549 cells , and lung cancer ( A549 ) evaluate BrMC Reversal P53 gene superfamily methylation experiments and theoretical basis , looking for the treatment of lung cancer drug candidate development prospects . Methods Cultured A549 cells . And colony forming assay to determination BrMC anchor - dependent inhibition of the in vitro culture of A549 cells growth role ; PI staining and flow cytometry (FCM) analysis of the rate of apoptosis ; conclusive evidence of DNA gel electrophoresis BrMC A549 cells induced apoptosis ; methylation The specific PCR BrMC reversal of the P53 gene hypermethylation status of A549 cells . Results and colony forming assay measurement results , BrMC (0.1,0.3,1.0,3.0,10.0,30.0 μmol / L) for 48 hours , the human lung carcinoma A549 cells colony inhibition rates were 7.9% , 19.7% , 28.1% , 39.8 %, 51.9%, 65.4%, IC50 value 7.6μmol / L . DNA agarose gel electrophoresis. \The PI staining FCM analysis found induced A549 apoptosis rate BrMC (0.3,3.0,30.0 μmol / L) were 2.8% ± 0.60 % , 5.1% ± 0.11 % , 19.8 % ± 1.74 % ; 10.0μmol / L BrMC ( 0,24 , 48,72 h) induced A549 apoptosis rates were 0.83% ± 0.13 % , 5.36 % ± 0.11 % , 8.78% ± 0.05 % , 22.67 % ± 1.16 % , which the 30.0μmol / L BrMC group apoptosis rate high in the blank control group ( P lt; 0.01 ) . Methylation specific PCR A549 cell P53 gene the hypermethylated , BrMC can reverse the P53 gene hypermethylation . Conclusion 1.BrMC inhibit the in vitro culture of human lung adenocarcinoma A549 cells in a concentration - dependent manner . 2.BrMC effectively induced apoptosis in human lung adenocarcinoma cell line A549 cells , a dose- and time-dependent manner . 3.BrMC growth inhibition and apoptosis in A549 to its reversal of the P53 gene hypermethylation related .
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