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The Experimental Research of Immunogenic Cell Expression Mediated by Low-dose Radiotherapy in Human Renal Clear Cell Carcinoma 786-0 Cell

Author: MaZhenFeng
Tutor: ZhaoChunLi
School: Hebei University
Course: Surgery
Keywords: Radiation therapy Calreticulin ( CRT ) MHC - I / II Class I molecules Immunogenic 786-0 Cell
CLC: R737.11
Type: Master's thesis
Year: 2011
Downloads: 8
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Abstract

Objective: To investigate small doses of radiation on human renal clear cell carcinoma 786-0 cell immunogenicity calreticulin (CRT), MHC-I and MHC-II expression amount, and to explore the impact of radiotherapy on the immunogenicity of the tumor cells, , to further improve the efficacy of cancer treatment to provide basic experimental data for better play synergistic anti-tumor effect of radiotherapy and immunotherapy. Methods: CCK-8 assay radiotherapy the 786-0 cell growth inhibition; different doses of radiation acting on 786-0 cells after 24 h by flow cytometry cell apoptosis was detected by indirect immunofluorescence and membrane surface of the CRT , expression of MHC-I and MHC-II molecules. Results: 1 CCK-8 test results: 0Gy, 6Gy, 12 Gy, 24 Gy four groups of irradiation on the 786-0 cell proliferation inhibition rate with increasing dose increased. The half inhibitory dose (IC50): 12Gy. 2 0Gy 6Gy, 12 Gy, 24 GY four groups irradiation on the the 786-0 cell surface expression of CRT: four different dose intervention of 786-0 cells after 24 hours, flow cytometry cell surface expression of CRT in the amount of , corresponding to the mean fluorescence intensity were: control group: CRT (0Gy) 13.32% ± 2.01%; treatment groups: the CRT (6Gy) 78.25% ± 5.83%; CRT (12Gy) 202.24% ± 16.47%; CRT (24Gy) 83.51 % ± 8.26%. Each group were compared with the control group twenty-two, there were significant differences (p lt; 0.001). To 12Gy group for the strongest, followed by 24Gy weakest at 6Gy group. With the increase of the dose, the mean fluorescence intensity of calreticulin first gradually increased, after a downward trend. The 12Gy group 6Gy group and 24Gy set twenty-two There were significant differences (P = LT; 0.05). 3 0Gy 6Gy, 12 Gy irradiation dose of 24 Gy four groups on the the 786-0 cell surface expression of MHC-I: four different dose intervention of 786-0 cells after 24 hours, flow cytometry cell surface MHC- I expression amount of the corresponding mean fluorescence intensity were: control group: MHC-I 8.24% ± 25%; treatment groups: MHC-I (6Gy) 16.26% ± 1.81%; MHC-I (12Gy) 27.39% ± 2.43%; MHC-I (24Gy) 48.72% ± 3.85%. Each experimental group compared to the control group or two, a significant difference (P values ??were lt; 0.05), and MHC-I expression increased with the dose increased gradually increased, showed a significant dose-dependent relationship. 4 0Gy 6Gy, 12 Gy irradiation dose of 24 Gy four groups 786-0 cell surface MHC-II expression :: four different dose intervention of 786-0 cells after 24 hours, flow cytometry cell surface MHC -II expression amount of the corresponding mean fluorescence intensity were: control group: MHC-II (0Gy) 9.07% ± 1.43%; treatment groups: MHC-II (6Gy) 11.46% ± 1.55%; CRT (12Gy) 23.82% ± 2.61%; MHC-II (24Gy) 31. 52% ± 2.63%. Each experimental group compared with the control group twenty-two, there were significant differences (P = LT; 0.05) With the irradiation dose increase MHC-II expression gradually increased, and the same was a significant dose-dependent relationship. Conclusion: 24 hours after radiotherapy, human renal clear cell carcinoma the 786-0 cell surface immunogenic molecular CRT, MHC-I, MHC-II upregulation of radiotherapy in addition to the direct killing effect on tumor cells, but also to induce the The immunogenic enhanced to create the conditions to improve the ability of immune cells recognize kidney tumor antigens and induce more powerful tumor-killing effect. 2 dose with CRT, MHC-I, MHC-II the 786-0 cell surface expression in renal cell carcinoma has some relevance, while lower doses of radiotherapy will be able to achieve this effect, indicating that small doses of radiation have been able to sufficient to induce 786-0 renal cell carcinoma tumor cells to produce immunogenic changes, suggesting that small doses of radiation can induce anti-kidney tumor immune response, thus avoiding the severe immunosuppression and high-dose radiotherapy for normal body organ tissue poison Vice injury. The results of this study suggest that: small doses of radiation can induce renal clear cell carcinoma the 786-0 cell membrane surface immunogenicity associated membrane molecules CRT, MHC-I and MHC-II expression increased, indicating that the radiation induction and strengthen the body to produce anti-kidney tumor the ability of the immune response, although the detailed mechanisms still need to continue to explore synergies, low-dose radiotherapy joint immunotherapy, it is possible to achieve a more effective anti-a new way for the treatment of renal tumors.

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