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Objective To observe the exogenous H2S (hydrogen sulfide, H2S) pheochromocytoma cells ( Pheochromocytoma cells , PC12 ) amyloid precursor protein / β- site amyloid protein ( amyloid precursor protein / β -amyloid , APP / Aβ ) metabolic pathway , and to explore the H2S to the metabolic pathways critical enzyme β- site amyloid precursor protein cleaving enzyme 1 (β-site amyloid precursor protein cleaving enzyme 1, BACE1) regulation of the expression . Methods using sodium hydrosulfide (sodium hydrosulfide, NaHS) exogenous H2S donor , experimental and blank control group , the NaHS 50μmol / L group , NaHS 100μmol / L group and NaHS 200μmol / L group , grouped by concentration process in PC12 cells 24h , the key protein APP APP metabolism process detected by Western blot , C99 , HARMONIZED SYSTEM OF QUALITY ASSESSMENT FOR expression , ELISA assay Aβ40 and Aβ42 protein levels in the cell culture medium and then detect cell RT-PCR and Western blot BACE1 mRNA and protein expression . Results 1.NaHS APP protein expression in the experimental concentration range has no effect , NaHS group compared with the control group , the difference was not significant sex ( P gt ; 0.05) . 2 with the control group protein ( the C99 : 0.937 ± 0.053 ; C83 : 0.427 ± 0.064 ; protein of Aβ40 : 43.594 ± 2.475 ; the Aβ42 : 36.812 ± 2.480 ) as compared with the increase in NaHS concentration each NaHS group order (C99: 0.655 ± 0.043,0.534 ± 0.015,0.254 ± 0.046; C83: 0.531 ± 0.020,0.737 ± 0.034,1.042 ± 0.050; Aβ40: 34.131 ± 2.873,26.051 ± 3.662,17.705 ± 1.713; Aβ42: 30.743 ± 1.225,21.537 ± 2.964,13.313 ± 3.664), showed a dose-dependent reduction of C99, Aβ40 and Aβ42 expression raised C83 expression among groups differences were statistically significant (P lt; 0.05), while on Aβ40/Aβ42 not affect ( P gt ; 0.05 ) . 3 with the control group ( BACE1 mRNA : 0.939 ± 0.036 ; BACE1 protein : 0.999 ± 0.041) compared to , NaHS in gene and protein level was dose-dependently reduced the the BACE1 expression of each the NaHS group of BACE1 expression order (BACE1 mRNA : 0.797 ± 0.105,0.513 ± 0.064,0.344 ± 0.061 ; the BACE1 protein : 0.638 ± 0.054,0.404 ± 0.085,0.251 ± 0.072 ) , the difference was statistically significant (P lt; 0.05) Conclusion Exogenous H2S down vitro culture C99, Aβ40 and Aβ42 key protein , APP metabolism PC12 cells raised the role of C83 expression , its lowered APP / Aβ pathway by down-regulating BACE1 expression occurred .
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