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The first part of ginsenoside Rd pilocarpine-induced epilepsy rat model of netrin-1 expression and neuronal apoptosis Objective: To investigate the ginsenosides of Rd (GSRd) on the dentate gyrus after status epilepticus in rats netrin-1 expression and hippocampal CA3 neurons apoptosis. Methods: lithium chloride - pilocarpine rat model of epilepsy. 30 healthy adult Wistar rats were randomly divided into three groups of epilepsy model the group, GSRd intervention group and the saline control group. Immunohistochemistry and TUNEL method was used to observe the dentate gyrus netrin-1 protein expression and hippocampal CA3 neurons apoptosis. The results: 1, of epilepsy model the group, GSRd intervention group and control group rats dentate gyrus 7d netrin-1 positive cells were between the treatment groups: 48.3 ± 5.0,25.7 ± 4.8,3.6 ± 1.5, df value = 2, F value = 273.9, P value = 0.000 30d netrin-1 positive cells were: 62.3 ± 4.8,31.6 ± 4.7,3.5 ± 1.8 between the treatment groups, df = 2, F = 242.9, P-value = 0.000. Rat dentate gyrus netrin-1 protein expression in epilepsy model group increased significantly compared with the saline control group, the difference was significant (P lt; 0.05); epilepsy model group, netrin-1 protein expression of GSRd intervention group significantly reduced (P lt; 0.05) 2, the epilepsy model groups, GSRd intervention group and normal saline control group rat hippocampal CA3 7d TUNEL positive cells were between the treatment groups: 16.0 ± 2.1,7.7 ± 1.8,6.6 ± 1.0, df value = 2, F = 54.3, P value = 0.000 30d by TUNEL-positive cells were: 13.6 ± 2.5,6.5 ± 1.4,5.5 ± 1.6 between the treatment groups, df = 2, F value = 45.2, P value = 0.000. Epilepsy model rats hippocampal CA3 region of TUNEL-positive cells was significantly higher than that in the control group (P lt; 0.05), the intervention group TUNEL positive cells than epilepsy model group decreased significantly (P lt; 0.05) GSRd. Conclusion: GSRd by downregulating the expression of netrin-1, and the reduced number of apoptotic neurons, which play a role in the protection of neurons. The second part of the match dynamically change the purpose of the netrin-1 expression and mossy fiber sprouting in pilocarpine model of epilepsy: To investigate the role of the nerve induced to factor netrin-1 in the epilepsy the state in hippocampal mossy fiber sprouting (MFS). Methods: lithium chloride - pilocarpine rat model of epilepsy, the use of of Timms staining and immunohistochemistry were used to detect mossy fiber sprouting and netrin-1 expression in the rat hippocampus. Results: epilepsy model rats in the first two weeks and four weeks, netrin-1 expression in hippocampal dentate gyrus than the saline control group increased significantly, and can see the mossy fiber sprouting through the granule cell layer of the dentate gyrus arrive within The molecular layer, and the formation of a dense laminar band. Conclusion: After status epilepticus in dentate gyrus netrin-1 expression increases, netrin-1 may be involved in the process of epilepsy after mossy fiber sprouting.
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