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Umbilical cord mesenchymal stem cells on the regulation of Th17 cells in patients with systemic lupus erythematosus

Author: LiuHuiQing
Tutor: SunLingYun
School: Nanjing Medical University
Course: Internal Medicine
Keywords: Lupus erythematosus Systemic Umbilical cord mesenchymal stem cells Transplant T lymphocytes Cytokines
CLC: R593.241
Type: Master's thesis
Year: 2011
Downloads: 46
Quote: 0
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Abstract


Objective To investigate the umbilical cord mesenchymal stem cells ( UC - MSCs ) in vitro and in vivo in patients with systemic lupus erythematosus ( SLE ) in PBMC CD3 CD8 - IL17A T lymphocytes and related cytokines regulation . The method of UC - MSCs transplantation in the treatment of 14 patients with SLE , observe the pre - and post - transplant clinical and laboratory indicators of change . Flow cytometry before transplantation in patients with SLE peripheral blood CD3 CD8-IL17A T cell percentage than normal if there is differences, and different time points after transplantation in patients with peripheral blood CD3 CD8-IL17A T cell percentage , ELISA determination of plasma IL - 6 , TGF-β, IL-17A, IL-22 expression levels . 10 patients with SLE peripheral blood mononuclear cells (PBMC) and UC - MSCs in different proportions in vitro cultured for 72 h and analyzed by flow cytometry in PBMC CD3 CD8 - IL17A percentage of T cells . UC - MSCs transplantation disease activity index score ( SLEDAI ) at 3 months ( 7.8 ± 1.2 ) and 6 months ( 6.9 ± 0.9 ) were significantly lower than before transplantation ( 10.4 ± 0.9 , p lt ; 0.05 ) ; 24 hour urine protein quantitative 6 months after transplantation decreased significantly ( 1489 ± 260 mg vs. 2454 ± 322 mg , p lt ; 0.05 ) ; serum albumin levels 1 month after transplantation ( 38.46 ± 1.52 g / l ) , 3 months ( 39.35 ± 1.58 g / L ) and 6 months (39.81 ± 1.82g / L) were significantly higher than before transplantation (31.63 ± 1.83g / L, p lt; 0.05), the complement C3 transplantation 1 month after ( 0.68 ± 0.05 g / L ) , 3 months ( 0.74 ± 0.05 g / L ) , 6 months ( 0.84 ± 0.05 g / L ) continues to rise . SLE patients with UC - MSCs transplantation of peripheral blood CD3 CD8 - IL17A T lymphocyte percentage ( 1.69 ± 1.13 ) than normal ( 0.03 ± 0.02 ) was significantly higher ( p lt ; 0.05 ) , 1 month after the UC - MSCs transplantation ( 0.73 ± 0.60 , n = 13 ) and 6 months ( 0.52 ± 0.40 , n = 10 ) , patients with peripheral blood CD3 the CD8 - IL17A T lymphocyte percentage decreased significantly ( p lt ; 0.05 ) . UC - MSCs and patients with SLE PBMC co-culture can significantly cut CD3 the CD8 - IL17A T lymphocyte percentage ( P lt ; 0.05 ) , but no dose-dependent manner . UC - MSCs transplantation in patients with plasma IL - 6 , TGF-beta , IL - 17A , IL - 22 level before transplantation ratio, the difference was not statistically significant ( p GT ; 0.05 ) Conclusion UC - MSCs transplantation in the treatment of SLE , SLE patients after transplantation of PBMC , T lymphocytes , CD3 CD8 - IL17A levels were significantly decreased .

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CLC: > Medicine, health > Internal Medicine > Systemic disease > Autoimmune diseases > Autoimmune diseases, connective tissue disease > Lupus erythematosus > Systemic lupus erythematosus
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