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Effects of Angiotensin Ⅱ and Telmisartan on Transient Outward Potassium and L-Type Calcium Currents in Sprague-Dawley Rat Atrial Myocytes

Author: LongYi
Tutor: YinYueHui
School: Chongqing Medical University
Course: Internal Medicine
Keywords: AngiotensinⅡ Telmisartan Transient Outward Potassium Current L-Type Calcium Current Patch Clamp
CLC: R541.75
Type: Master's thesis
Year: 2011
Downloads: 23
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Abstract


Objective:To explore possible electrophysiological mechanisms of single atrial myocyte by evaluating the effects of angiotensinⅡ（AngⅡ） and telmisartan on transient outward potassium currents(Ito) and L-type calcium currents (ICa-L) in Sprague-Dawley rats.Methods:Single atrial myocyte of SD rats was obtained by enzymatic dissociation method. The whole cell patch-clamp recording technique was used to record the change of Ito and ICa-L by intervening of AngⅡ, telmisartan and AngⅡplus telmisartan respectively. Experimental groups:⑴AngⅡgroup: cells were perfused with bath solution containing AngⅡ（0.1μmol/L）;⑵Telmisartan group: cells were perfused with bath solution containing telmisartan （0.01μmol/L）;⑶Combined group, myocytes were perfused with bath solution containing AngⅡ（0.1μmol/L） and telmisartan （0.01μmol/L）.Results : Compared with baseline value, AngⅡ（0.1μmol/L）, Telmisartan（0.01μmol/L） and AngⅡplus Telmisartan group significantly decreased the peak density of Ito in SD rat atrial myocytes （22.48±2.75 vs. 15.71±2.06 pA/pF, P<0.01）, （24.16±2.36 vs. 16.15±1.82 pA/pF, P<0.01） and （24.41±2.27 vs. 21.35±1.46 pA/pF, P<0.05） respectively. AngⅡ（0.1μmol/L） significantly increased the peak density of ICa-L in SD rat atrial myocytes （-4.51±0.38 vs. -5.16±0.29 pA/pF, P<0.01）. Telmisartan （0.01μmol/L） had no significant effects on ICa-L in the rat atrial myocytes （-4.35±0.27 vs. -4.29±0.34 pA/pF, P>0.05）, but it could antagonize the effects of AngⅡ. In the AngⅡcombined telmisartan group, the peak density of ICa-L was（-4.08±0.28 vs. -4.20±0.31 pA/pF, P>0.05）, which was significantly different from that of AngⅡgroup （P<0.05）.Conclusion:AngⅡand telmisartan had directly electrophysiological effects on SD rat atrial myocytes as well as telmisartan had antagonist effects on AngⅡat the level of angiotensin receptor.

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CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Arrhythmia > Atrial fibrillation and atrial flutter
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