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Endometrium Morphologic Analysis and Expressions and Function of Activin A and Inhibin α in the Endometrium of Peri-implantation in Mice

Author: LiXiaoLan
Tutor: ChenXiaoRong
School: Anhui Medical University,
Course: Human Anatomy and Embryology
Keywords: Morphology Activin A Inhibinα Implanted Endometrial Mice
CLC: R321
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 0
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Abstract


Objective: To investigate the expression of Activin A and Inhibinα in the mouse endometrium regular changes and their impact on the implantation of the blastocyst, and explore its role in the mice embryo implantation process. Methods: adult nulliparous female Kunming mice to study, estrus females were normal male mice and vasectomy males 2:1 in the same cage, the next morning found overcast tied in mind is true (false ) pregnant 1d. 2 ~ 6d really pregnant to and cervical dislocation pseudopregnant mice were sacrificed, laparotomy whichever estrus uterus and take the non-pregnant mouse uterus as a control, and more than 4% paraformaldehyde fixed HE staining really pregnant and pseudopregnant light microscopy in mouse endometrium; electron microscopy really pregnant mice endometrial ultrastructural changes; immunohistochemical SP method to detect endometrial Activin A and Inhibinα, expression; computer image analysis of immunohistochemical staining results; in pregnant 4d morning the 9AM mouse uterine horn were injected with Activin A antibody and saline in pregnant the 8d observed implantation blastocyst number compared with the normal control group, Activin A blastocyst implantation. Results: (1) HE staining results: true dynamic trend was pregnant mouse endometrium of 2 ~ 6d, which increased the glands in the lamina propria of 4 ~ 5d, endometrial edema, stromal cell hypertrophy, the gap widened, vascular The hyperplasia significant. Transmission electron microscopy results: true pregnant 2 ~ 6d mouse endometrial luminal epithelium, glandular epithelial and stromal cells, organelles estrus when compared with non-pregnancy-rich, 4 ~ 5d endometrial luminal epithelium and glandular epithelium within rich in mitochondria, rough endoplasmic reticulum, secretory granules, mast stromal cells rich in glycogen, lipid droplets. Immunohistochemical results: true pregnant ~~ 6d of activin A expression in endometrial luminal epithelium, glandular epithelium and stroma cytoplasm, the strongest expression of 4 ~ 5d; Inhibinα mainly expressed in the endometrium of luminal epithelium and glandular epithelium cytoplasm, the strongest expression of 4 ~ 5d; the pseudopregnant 2 ~~ 6d of activin A and the Inhibinα expression in endometrial and really pregnant group was significantly different. Computer image analysis system calculated the Activin A Inhibinα 2 ~ 6d pregnant endometrium mean optical density (MOD) in the real value, the SPSS software, both in pregnant 2 ~ 6d endometrial expression has a positive correlation. 5. In the of preimplantation pregnant 4d9AM uterine horn injection Activin A antibodies in pregnant 8d observed number of blastocyst implantation results the blastocyst implantation significantly reduced the number after the injection of Activin A antibody, a significant difference (P lt; 0.05). Conclusion: 1. The true pregnant and false pregnant 2 ~ 6d mouse endometrial morphology are dynamic trend in pregnancy 4-5d changes most significantly. 2. Really pregnant 2 ~ 6d mouse endometrial cavity epithelium, glandular epithelium and stroma cells continued to express Activin A, suggesting that Activin A may play an important role in the mouse embryo implantation process. 3. Really pregnant the sustained expression 2 ~ 6d mouse endometrial cavity epithelial and glandular epithelium Inhibinα, prompted Inhibinα may play an important role in the mouse embryo implantation process. 4 pseudopregnant 2 ~ 6d expression pattern in mouse endometrium is significantly different and really pregnant, prompted Activin A and Inhibinα, expressed not only by the impact of maternal hormones, and embryo implantation related. The 5. Activin A and Inhibinα really pregnant 2 ~ 6d expression in mouse endometrium positive correlation, the two may be involved in the process of embryo implantation collaborative. With Activin A antibody in mouse blastocyst the uterine horns injection of preimplantation blastocyst implantation significantly reduced, suggesting that Activin A may play an important role in the blastocyst implantation.

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CLC: > Medicine, health > Basic Medical > Human morphology > Human embryology ( human embryology,embryology )
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