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Study on the Whole Peptidoglycan of Bifidbacterium under Treatment of Food Allergic Mice and Effect on Regulatory T Cells

Author: ChenYuMei
Tutor: ChengZuo
School: Chongqing Medical University
Course: Pediatrics
Keywords: Whole peptidoglycan Food allergies Regulatory T cells
CLC: R392.12
Type: Master's thesis
Year: 2011
Downloads: 55
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Abstract


Objective: food allergy in mice as an animal model, fed with bifidobacterial whole peptidoglycan (Whole Peptidoglycan WPG) to observe the effects of its treatment of food allergies, as well as the role of regulatory T cells; explore Bifidobacterium prevention allergic diseases may be a mechanism to provide for the probiotic their further development and utilization of bio-active ingredients. Method: object: eggs 24 to feed 4 to 6-week-old SPF level Balb / c female mice were randomly divided into three groups, each of eight. Take 2 group to establish a food allergy model, respectively: OVA sensitized positive control group (OVA group) and OVA excited fed with bifidobacteria the WPG treatment group (T group); while normal saline negative control group (C ). Methods: (1) observation of symptoms; (2) HE staining was used to observe the morphological changes of the intestinal tissue; (3) ELISA assay serum OVA-specific IgE levels; (4) Flow cytometric analysis of spleen mononuclear CD4 CD25 cell suspension regulatory changes in the number of T cells; (5) ELISA assay serum level of IL-10 and TGF-β. Results: (1) of WPG OVA-mediated immediate allergic alleviate T group, only 12% (1/8) mice suffered from diarrhea and diarrhea degree is significantly reduced compared to the OVA group and the OVA mice, 87.5% (7/8) suffered from diarrhea; higher than the average weight gain of T mice OVA group (P lt; 0.05), HE staining focal necrosis, shedding of epithelial cells in the small intestine villi, lamina propria inflammatory cells infiltration significantly improved, OVA-specific IgE levels were significantly lower, the difference was statistically significant (P lt; 0.05); (2) OVA group compared with the C group, Foxp3-positive cells in the mouse spleen mononuclear cell suspensions (Foxp3 CD4 CD25 regulatory T cells) accounted CD4 CD25 T cells were significantly reduce (P lt; 0.01), serum IL-10 and TGF-β levels were decreased, and the difference had statistically significant (P lt; 0.05); (3) T group CD4 CD25 T cells in the mouse spleen mononuclear cell suspensions accounted for CD4 T cell levels higher than the OVA group, the difference was statistically significant difference (P lt; 0.05), compared with C group of normal mice showed no significant difference (P gt CD4 CD25 T cells; 0.05); the T mice spleen cells of Foxp3-positive cells (Foxp3 CD4 CD25 regulatory T cells) accounted content compared with the OVA group was significantly higher (P lt; 0.01). T mice serum IL-10 levels higher than the OVA group (P lt; 0.05); TGF-β levels were significantly higher than the OVA group (P lt; 0.01). Conclusion: (1) of WPG and Bifidobacterium bacteria OVA-sensitized mice have the same therapeutic effect: You can ease the symptoms of acute seizures in mice, reduce the incidence of food allergies rate, reduce the pathological changes of intestinal inflammation; (2) of WPG in the treatment of food allergies increase the number of CD4 CD25 regulatory T cells, stimulation of the cytokines IL-10 and TGF-β secretion regulate Th1/Th2 balance. May be one of the mechanisms of the Bifidobacterium control food allergies.

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