Dissertation > Excellent graduate degree dissertation topics show

The Relation of the Activity Astrocyte and the Expression of EGFR

Author: GuoZhiHui
Tutor: WuHeng
School: Nanhua University
Course: Department of Neurology
Keywords: Astrocyte activation Fibrillary acidic protein Genistein Epidermal Growth Factor Receptor
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 32
Quote: 0
Read: Download Dissertation

Abstract


Objective To study the activation of astrocytes (Astrocyte, Ast) expression of the epidermal growth factor receptor (epithelialgrowth factor receptor, EGFR) , and explore the EGFR played a key role in the activation of astrocytes . Shaking culture method combined with differential activation group affixed wall separation and purification of cultured astrocytes , the second generation of astrocytes will be divided into a control group , and the suppression group : routine containing 10? S DMEM medium 24h after astrocytes cells as the control group , with 20ng/ml ciliary neurotrophic factor , 10 ? astrocytes S in DMEM culture medium 24 h after activation group cells containing 30μmol / L effect of Genistein 10 ? S DMEM medium role in the activation group cells incubated for 24 h after cell as inhibitory cells . The observed changes in the morphology of the cells by immunofluorescence ; semi - quantitative RT-PCR analysis fibrillary acidic protein expression between cells ( glial fibrillary Irregularity in the protein , GFAP) in mRNA and EGFR mRNA expression . Activation group and the control group compared with the results the increase in the number of astrocytes cells , larger cell body processes increased variable length reticulation ; RT-PCR shows not only GFAP mRNA expression increased the , EGFR mMRA expression also significantly increased , with the control group were significantly different ( P <0.01 ) ; application EGFR inhibitor Genistein intervention , compared with the activation of the group , no longer increase the number of astrocytes smaller cell bodies , processes reduce shortened astrocyte activation suppression of GFAP mRNA expression decreased activation group were significantly different ( P <0.01 ) . Conclusion : 1 . Astrocytes activated EGFR expression is significantly upregulated by inhibiting EGFR expression ; 2.Genistein astrocyte activation can be inhibited .

Related Dissertations

  1. Expression and clinical significance of EGFR and downstream signaling molecules in ovarian cancer,R737.31
  2. Preliminary Study on the Expression of Epidermal Growth Factor and Epidermal Growth Factor Receptor in Similar Clinical Stage But Different Prognosis Tongue Squamous Cell Carcinoma,R739.86
  3. Changes in the Expression of Transforming Growth Factor Alpha and Epidermal Growth Factor Receptor on Portal Hypertensive Gastropathy Healing in Patients,R575.2
  4. Transcription Factor Pax3 Negatively Regulate the Expression of Glial Fibrillary Acidic Protein Gene,Q42
  5. Molecular Simulations of Epidermal Growth Factor Receptors (EGFR/ErbB2),R917
  6. Different Formulations of Gingival Retraction on the Impact of Gingival Tissue,R783.3
  7. The Role of MicroRNAs and Target Gene in Uveal Melanoma and Gastric Cancer Development and Progression,R735.2
  8. Effects of ERK1/2 Signaling on the Estrogenic Activities of Genistein and Equol,R737.9
  9. Effect of HER-2 Gene on Anthracycline Adjuvant Chemotherapy Efficacy in Breast Cancer,R737.9
  10. The Study on the Effects of Using Different Doses of Genistein During Pregnancy on Vascular Endothelial Growth Factor Expression in the Offsprings of Mice Mammary Tumors,R737.9
  11. Expression and Significance of EGFR、MMP-9、and Ki67 in Human Glioma,R739.41
  12. DHA Attenuates Lipopolysaccharide-induced White Matter Injury in the Neonatal Rat Brain,R742.3
  13. Chronic mechanical pressure on MUC5AC expression and signal transduction mechanism,R56
  14. The Study on the Changes of Calcium Ion Concentration and Gene Expression in Mesenchymal Stem Cells During Their Differentiating into Neuron-like Cells,R329
  15. The Initial Study on the Effects of Genistein on the Proliferation and Invasion of Human Tongue Carcinoma Cell Line Tca8113 in Vitro,R739.8
  16. Correlation Between Epidermal Growth Factor Receptor and Canceration of Oral Leukoplakia,R739.8
  17. The Effect of Genistein on Uveal Melonama and the meta Analysis of Age-related Macular Degeneration Risk Factor,R739.7
  18. The Study of Biological Parameters Associated with the Sensitivity to Chemoradiotherapy of ESCC,R735.1
  19. Effect of Genistein on Breast Cancer During Pregnancy (MMTV)-ErbB-2 Transgenic Mouse Expression of p53,R737.9
  20. A Preliminary Study on the Correlation Between Hif-1α and Gefitinib-resistant,R734.2
  21. Promotion Apoptosis Effect and Its Mechanism of genistein Combined with 5-Fu on Human Colon Carcinoma Cell,R735.3

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile