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The Research of Tea Seed Polysaccharide from Cold Squeeze Leaching Camellia Cake

Author: HuangWei
Tutor: HeDongPing
School: Wuhan Polytechnic University
Course: Cereals, Oils and Vegetable Protein Engineering
Keywords: camellia polysaccharide camellia seed cold squeeze leaching cake
CLC: TS229
Type: Master's thesis
Year: 2011
Downloads: 18
Quote: 0
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Camellia is a unique tree oil crops in China. Camellia forest area of the country 400hm2, mainly in the south of Hunan, Jiangxi and other 16 provinces (regions) more than 1,100 counties, accounting for 80% of woody oil over the area, our annual output of more than 100 tons of tea seed oil. Preparation of tea oil camellia fruit is nuts, have a high economic value and food value. Utilization of a long history of tea seed meal, with the rapid development of new technologies, the value of polysaccharides gradually taken by the people, full use of our resources of oil tea seed meal, tea seed oil-depth study of polysaccharides, to speed up the transformation theory, to achieve resource direction to the industry in promoting sustainable economic development and comprehensive utilization of natural resources to create economic value is of great significance.In this paper, camellia seed meal as the research object shelling leaching, leaching extraction of methanol, were designed for a single factor and orthogonal experiments were optimized to determine the optimum methanol extraction, has been tea seed polysaccharide, The tea seed obtained on removal of protein and polysaccharide solution bleaching, are purified tea seed polysaccharide. Antioxidant activity while doing their research.The main conclusions of this paper is as follows:(1) Determine the phenol - sulfuric acid method of optimization of the color reaction conditions: reaction temperature 100℃, reaction time of 20min, detection wavelength 490nm. Determined by the average sample yield was obtained 97.96%, relative standard deviation (RSD) was 2.73%. This method is accurate, reproducible, sensitive, can serve as a laboratory determination of camellia seed polysaccharide content analysis.(2) This paper was extracted by methanol extraction of oil tea seed polysaccharide extraction and optimization of the extraction process. Extraction process in the study, with methanol - water as extraction solution, by studying the material to liquid methanol, solvent concentration, extraction temperature and extraction time of four factors, combined with single factor and orthogonal tests, to determine the best extraction conditions were: liquid ratio 1:10 (W / V); solvent concentration of 80%; extraction temperature 60℃; extraction time 3h. At this point polysaccharides extracted rate was 3.92%.(3) Respectively Sevage method, TCA (trichl oroacetic acid) method, HCl method deproteinized polysaccharide solution of tea seed, tea seed polysaccharides on the effect of removing protein were studied. Sevage method in which the loss rate of 37.3% polysaccharide, protein removal rate was 83.7%; TCA method polysaccharide loss rate of 18.9%, protein removal rate was 74.3%; the HCl method polysaccharide loss was 16.6%, removal rate was 52.1%; the test selection method for the TCA primary method of removing protein polysaccharides.(4) Using activated carbon, hydrogen peroxide and Diatomite on the decolorization of oil tea seed polysaccharide tested, comprehensive comparison of selected polysaccharide carbon decolorizing agent for the tea seed oil, and were single factor experiments, the pH 6.0, 2% active carbon, the reaction time of 50min, temperature 60℃, the decolorization rate reached 68.5% polysaccharides, polysaccharide retention rate of 78.3%.(5) camellia seed polysaccharide were measured antioxidant capacity, confirmed the camellia seed polysaccharide O2-·and·OH have a clear role, and the concentration of polysaccharides positive correlation. Using the pyrogallol method, when the polysaccharide concentration 2% ,·OH scavenging rate was 83.7%; Fenton oxidation measured, when the polysaccharide concentration of 1%, the O2-·clearance rate of 49.1%.

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