Dissertation > Excellent graduate degree dissertation topics show

Genetically Encoded Fluorescent Sensor for Copper Ion

Author: DengMinZuo
Tutor: HuangQingChun；YangZuo
School: East China University of Science and Technology
Course: Pesticides
Keywords: Copper ions CueR YFP Fluorescent probe
CLC: Q78
Type: Master's thesis
Year: 2011
Downloads: 95
Quote: 0
Read: Download Dissertation

Abstract


Metal ions in a wide range of life processes in vivo , they maintain proteins, nucleic acids and other biological macromolecules of the basic structure, to achieve normal life functions of the body plays a very important role. Copper is the third in vivo levels of trace metals , the body for the intracellular concentration of copper ions have a very strict regulatory mechanism of copper excess or deficiency can lead to disease. The copper ions are often used pesticides fungicides for inhibiting the growth of bacteria and fungi , but improper use can cause injury and even prevention through bioaccumulation directly endanger human health or the environment and therefore the organism of copper ions detection has important applications, it has long been sought in living cells can be detected with high resolution copper ion distribution methods . In this thesis, a copper ion regulation from Escherichia coli transcriptional promoter CueR protein and yellow fluorescent protein fusion constructs can respond to copper ions fluorescent protein probes CuFP. By means of genetic engineering to further probe the gene deletion mutagenesis and obtained a series of mutants. We use a variety of metal ions were titrated and studied these CuFP fluorescent protein probes the changing nature of the final screening with copper ions that can respond to specific fluorescent protein probes and silver ions are able to respond to specific fluorescence protein probes. This thesis probes will be used to obtain intracellular copper ions and silver ion detection, or pesticide residues and heavy metal contamination is detected.

Related Dissertations

  1. Expression Analysis and Localization of OsDMI3 Gene Induced by Abscisic Acid,S511
  2. Studies on Genotoxicity of Two Novel Contraceptive Materials,R114
  3. EGSB-MBR- ozone treatment process wastewater bamboo products,X798
  4. Study on Cu (Ⅱ)-MMT and Effect of the Avian Broilers Feed by Cu (Ⅱ)-MMT,S831.5
  5. DNA Biosensors for Point Mutation Identification and a Novel Sensing Interface Fabrication,Q75
  6. Reaction of Aminophenylbenzimidazoles and Synthesis of New Fluorescent Compounds,O626.23
  7. The Synthesis and Applications of Coumarin-7-Boronate Fluorescent Probe,Q946
  8. Synthesis, Structure Characterization and Optical Properties Research of Pyrazoline Derivatives as Well as Zn2+ Fluorescent Sensors Exploration,O626
  9. Fabrication and Properties Research of Novel Fluorescent Nanoparticles for Cupric Ion Detection,TB383.1
  10. The Study of Synthesis and Inclustion Behaviors of Several Cyclodextrin Derivatives with Drug Molecules,TQ460.1
  11. Gold Nanoparticles Quenching Fluorescence Probe for Glucose Detection and Cell Imaging Based on Fluorescence Resonance Energy Transfer (fret),Q503
  12. Fluorescent probe method to study the mechanism of action of the drug and biological macromolecules and their determination,R346
  13. Synthesis and Application of a New Luminescent β-Diketonate-Europium(Ⅲ) Complex,O627.3
  14. New amine fluorescent reagents in high-performance liquid - Mass,O657.72
  15. Fluorescent Probes for neurotransmitters , fatty acids Determination and Application,O657.72
  16. Study on Preparation of Soy Oligo-peptide, Function of ACE Inhibitory Activity and Metal-chelate,TS201.2
  17. Hydroxycinnamic Acids Act as the DNA-cleaving Agents in the Presence of Cu(Ⅱ) Ions: Mechanism, Structure-activity Relationship and Biological Implication,Q504
  18. Syntheses and Applications of Dendrimer-nanocomposites,TB383.1
  19. SECM-SPR combined with technology research sulfur protein and metal ions interactions,Q51
  20. Blue fox parvovirus strains Tai'an molecular characteristics and its TaqMan quantitative PCR method for detection,S852.65

CLC: > Biological Sciences > Molecular Biology > Genetic engineering (genetic engineering)
© 2012 www.DissertationTopic.Net  Mobile