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Folate receptor targeted fluorescent silicon nano- carrier synthesis , characterization and cell transport studies

Author: LouChangChun
Tutor: LiuZuoYao
School: University of Electronic Science and Technology
Course: Biophysics
Keywords: Nanoparticles MPS Folate Target delivery
CLC: TB383.1
Type: Master's thesis
Year: 2011
Downloads: 108
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Abstract


Nanoparticle is emerging as an ideal carrier for gene/drug delivery, which could be used for targeting therapy and optical imaging through various chemical modification methods. Nanoparticle has attracted considerable attention for their unique advantages. Silica has been employed in material sciences and engineering for many years. It is a versatile and benign material due to its biocompatibility and low toxicity. In this study, we used 3-mercaptopropyltrimethoxysilane (MPS) as a unique silica source to successfully synthesize a novel type of inorganic silica nanoparticles by modified St?ber method. This method solved the disadvantages of traditional method that the silica nanoparticles could not be easily modified. Our data showed that the mean diameter of nanoparticles ranged from 200 to 2000 nm by controling MPS concentration. Fluorescein isothiocyanate (FITC) could be doped into nanoparticles and conjugated to the surface of nanoparticles. Meanwhile, we can also link folate to the nanoparticles through poly(ethylene glycol) (NH2-PEG-Maleimide), thereby sythesizing a multifunctional nanoparticls for targeting delivery through the interaction of folate and its receptor. Fluorospectrophotometer and Fourier transform infrared spectroscopy were used to characterize the novel nanoparticles. Quantititive analysis of transfection in different cells showed that the delivery efficiency of KB cells (a folate receptor over-expressing cell line) is 6-fold higher than that of A549 cells (a folate receptor deficient cell line). The delivery efficiency of KB cells decreased significantly through adding the free folate receptor in the medium. The study showed that the nanoparticles could be targeted delivery into KB cells overexpressing folate receptors. Finally, we studied the endocytosis mechanism by several inhibitors of endocyrosis-related, confirming the clathrin played an important role in nanoparticles phagocytosis. Subsequently, the silica nanoparticles possessed the predominance of structural stability, fluorescence labeling and targeting. It is concluded that these silica nanoparticles have high potential in the optical imaging and targeting gene delivery of the tumor or artherosclerosis.

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CLC: > Industrial Technology > General industrial technology > Materials science and engineering > Special structural materials
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