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Objective: phorbol esters tetracyclic diterpenoid compounds is a physiological activity significantly , but its structure is complex , the the synthetic phorbol maternal structure is also very difficult . For further development and utilization of the experiment phorbol Extraction and purification of study , and to lay the foundation for further study later . Method: First, the principle of hydrolysis of the ester , crotonic (Croton tiglium Linn) oil in phorbol ester hydrolysis of phorbol then isolated and purified by extraction , column chromatography, recrystallization, etc. means prepared phorbol by UV , IR , ESI -MS , 1 to H - NMR and ( 13 ) ~~ C -NMR and other methods confirmatory structure and determined by HPLC purity, phorbol reference substance . Then establish RP - HPLC method for the determination of croton seed kernels in phorbol content . Finally the Orthogonal Test croton oil , phorbol ester hydrolysis process , extraction and separation and purification . Results : prepared phorbol purity greater than 98.5% , as a reference substance , the the Croton species kernels the phorbol average content of 0.18% , the average recovery was 96.40% , RSD 1.03% . Preferably with alkaline methanol , hydrolysis of 6 - fold amount of 10 h at 25 ℃ hydrolysis 1 , from the highest yield of the croton oil obtained phorbol by orthogonal test . The use of solvent extraction, column chromatography on silica gel , solvent recrystallization , and other means of separation and purification can be prepared in a higher purity phorbol . Conclusion: This study developed a method of extraction and purification of phorbol from croton , simple technology and equipment without special requirements , laid the foundation for the further study of large-scale industrial preparation and phorbol .
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