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Preparation of Porous Chitosan Magnetic Microspheres and Study on Magnetic Microspheres Immobilized Xylanase

Author: ZhangLiPing
Tutor: HanDeYan
School: Hubei Normal University,
Course: Analytical Chemistry
Keywords: Porous Chitosan Magnetic Microspheres Chitosan Magnetic Microspheres Copper ions Bovine serum albumin Xylanase Immobilized enzyme
CLC: O636.1
Type: Master's thesis
Year: 2011
Downloads: 145
Quote: 0
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Abstract


Magnetic polymer microspheres as a new type of functional materials, because of its unique superparamagnetic rich and polymer surface functional group characteristics, has been in biological chemistry, cytology, biomedical engineering and other fields has been widely used. Porous Polymer Magnetic Microspheres research in this field is one of the hot, porous polymeric microspheres unique porous structure of the microspheres can increase the specific surface area, increasing the microspheres opportunities for contact with the reactants, increase the use of efficiency. The main contents of this paper is porous chitosan magnetic microspheres (Porous Chitosan Magnetic Microspheres, PCMM) microspheres preparation and protein adsorption, metal ion adsorption and enzyme immobilization in the application: 1. Choose CaCO 3 porogen to nano-Fe is the magnetic core, do epichlorohydrin crosslinker, Span-80 do emulsifiers, liquid paraffin as the dispersion medium, using inverse suspension embedding prepared PCMM; Non-porous magnetic chitosan microspheres (Chitosan Magnetic Microspheres, CMM) preparation without adding CaCO 3 porogen. Scanning electron microscopy and transmission electron microscopy of both microspheres were characterized, from the results shown in SEM, PCMM surface roughness and a recess, effective to increase the specific surface area of ??microspheres. Studied two different microspheres by Simba Blue (Cibacron Blue F3G-A) modified bovine serum albumin (BSA) adsorption performance. The experimental results show that: when the concentration of bovine serum albumin 1.50 mg · mL -1 time, PCMM adsorption capacity of 131 mg · g -1 , CMM adsorption capacity of 117 mg · g -1 . 2. PCMM metal ions can also be used for wastewater treatment. In PCMM as a carrier in water Cu 2 adsorption properties were investigated. The experimental results showed that: in the same experimental conditions, PCMM for Cu 2 adsorption performance is much higher than the CMM; PCMM on Cu 2 The best adsorption time is 6 h, most good adsorption pH of 5.50, when Cu 2 at a concentration of 150 mg · L -1 time, PCMM for Cu 2 adsorption reached 95.0 mg · g -1 ; By drawing Langmuir isotherm and Freundlich isotherm can be seen, PCMM for Cu 2 adsorption are single molecules, are preferentially adsorbed; After eight repetitions After use, PCMM for Cu 2 adsorption capacity was reduced by 20% recycling rate is relatively high. 3 PCMM and CMM respectively as support, iminodiacetic acid (IDA) as a chelating agent chelate Cu 2 , was prepared immobilized xylanase. Studied two microspheres of different Cu 2 adsorption amount and concentration of chelating different Cu 2 The amount of influence on the enzyme activity; also discussed the amount of enzyme, pH value, time immobilized xylanase activity of. The results showed that the two microspheres immobilized xylanase optimum pH between 5.0-5.5 are the optimum temperatures were 50 ° C, Km values ??were 0.0722 mg · mL -1 and 0.0557 mg · mL -1 . Magnetic chitosan immobilized and free xylanase (Free Xylanase) compared to heat, pH stability, and so have a greater improvement; porous chitosan magnetic microspheres immobilized enzyme (Porous Chitosan Magnetic Microspheres Immobilized Enzyme, PCMM-IE) is also superior to the nature of the non-porous magnetic chitosan microspheres immobilized enzyme (Chitosan Magnetic Microspheres Immobilized Enzyme, CMM-IE); the two magnetic chitosan microspheres immobilized enzyme for degrading rice shell xylan, results showed that: PCMM-IE is not only more stable than CMM-IE, but also in the experiments on the degradation of xylan is also superior to CMM-IE, so immobilized xylanase especially porous shell polysaccharide immobilized xylanase magnetic applications with a wide range in the foreground.

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CLC: > Mathematical sciences and chemical > Chemistry > Polymer chemistry ( polymer ) > Natural polymers ( polymer ) > Polymer polysaccharides
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