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The dry Poria 93% Pachymaran contain small amounts of branched (1 → 3)-beta-D glucan, insoluble in water, significantly affect its activity and application of results. This paper aims to chemical, physical and biological methods, Pachymaran degradation of the preparation of water-soluble oligosaccharides, thereby enhancing its physiological activity. This article was prepared by the following three methods Poria oligosaccharides. The first method is the Poria the carboxymethyl prepared carboxymethylpachymaran, then enzymatic, to investigate the source of Trichoderma viride cellulase (cellulase), Plants composite hydrolase (Viscozyme L), beta-glucanase, Trichoderma reesei, the source of cellulose hydrolysis enzymes (cellulases) Carboxymethylpachymaran oligosaccharides effect. The Poria carboxymethyl process: Poria powder dispersed in 80% ethanol, solid-liquid ratio 1:20 (M: V), join 1.5mol/LNaOH (dissolved in 80% ethanol concentration, the same below), alkali at 55 ° C of 2h, in 0.75mol / L chloroacetic acid etherification 5h, 91% of Poria powder can converted to water-soluble carboxymethyl Pachymaran. Insoluble precipitate fraction again carboxymethylcellulose, 62% may continue to dissolve. After two carboxymethylation the, Poria powder dissolution rate of up to 96%. Four enzymes can degrade the Poria carboxymethyl polysaccharide, the the resulting oligosaccharides of polymerization degree of about 10, wherein the the hydrolase cellulase and plants composite degradation effect preferably, 2 to 3% of the added amount of carboxymethylcellulose The degree of polymerization of methyl Pachymaran reduced to 8-9, but Carboxymethylpachymaran oligosaccharides enzyme inhibition can not continue to enzymatic hydrolysis. The second method is by acid degradation, combined with the high temperature and pressure, preparing Carboxymethylpachymaran oligosaccharides. Compare the impact of acid type, temperature, and acid concentration on the degradation effect. Among them, the peracetic acid degradation Carboxymethylpachymaran the same time, the bleaching effect, better color of the product. Degradation between strong and weak acid, strong regulation. Under heat and pressure, to adjust the amount of peracetic acid for the 0.1 mol / L to 0.3mol / L, hydrolysis 2H, may be prepared by a different degree of polymerization degree of polymerization of 3 to 10 Carboxymethylpachymaran oligosaccharides. The third method is to use high temperature and high pressure and peracetic acid degradation of the polysaccharide Poria powder extraction and degradation. Under the action of temperature, pressure, and peracetic acid, to promote the dissolution Pachymaran and degradation, and to adjust the peracetic acid concentration of 0.05-0.3mol / L, 2h under high temperature and high pressure, may be prepared by a polymerization degree of 6 or less of the oligosaccharide , yield more than 83%. Poria the oligosaccharides and Carboxymethylpachymaran oligosaccharides proliferation of bifidobacteria in vitro. The results indicate that the The Poria oligosaccharides promote bifidobacteria preferably, allows the culture medium pH value decreased from 7 to 4.5; Carboxymethylpachymaran oligosaccharides weak proliferation of Bifidobacterium can culture broth a decrease in pH to 6. Comparing the two bacterial cell mass in the culture medium, and the former is approximately 3 times that of the latter.
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