Dissertation > Excellent graduate degree dissertation topics show

Study on the Hereditary Characteristics and Influences to the Soil Bacteriums of the Rice Offsprings Transferred 3α-HSD Gene

Author: WangZuoXin
Tutor: PanDaRen
School: Fujian Agriculture and Forestry University
Course: Cell Biology
Keywords: 3α-HSD gene Agronomic traits Quality Traits Bacterial
CLC: S511
Type: Master's thesis
Year: 2011
Downloads: 9
Quote: 1
Read: Download Dissertation

Abstract

Agrobacterium-mediated transfer 3a-HSD gene of rice test material using molecular techniques and agronomic traits and quality traits examine, from a time and space perspective, the detection of transfected genes offspring's genetic characteristics; transgenic rice planting soil separation to get carbon sources of bacteria populations identified cholesterol the study transgenic rice of planting environment bacterial populations. Stability of turn 3α-HSD gene in rice offspring genetic traits and growing environmental security for this transgenic rice breeding value do the preliminary study. Now the main results are as follows: 1. Transit 3α-HSD gene in rice T ₄, T ₅, T ₆ generation data analysis can seen progeny transgenic positive plants basic stability of the frequency of occurrence, the average can reach more than 90%, indicating that the gene can be stably inherited; are detected in roots, pollen and grain to a target gene, the target gene in various parts of the rice stable. Transgenic 3α-HSD gene tillering stage, booting and heading stage test results show that the roots of the target protein in stable trend gradually with the growth and development of inter-generational, T ₅ generation and T ₆ generation difference was not significant. Slowly increased with the growth of content, the highest concentrations of heading stage, an average of up to 2.56μg / g tillering stage lowest content as low as 0.79μg / g. Performance between different strains of generations, can stabilize genetic lines express the amount of average to 1.79μg / g, the expression levels of the 5 strains lowest average for 1.389μg / g; pollen and grain the content of the target protein is relatively small, in units of ng / g, an order of magnitude. The pollen target protein T5 substituting, and T6 substituting no significant difference, that is gradually stabilized. Purpose of grain protein content in the stability trend lines are good overall relatively high content of strains. Quality traits analysis showed that the soluble starch grains with soluble protein content compared to the control are reduced the sitosterol content is also reduced compared with the control, indicating that the 3α-HSD gene expression product degradation sitosterol can prove microbial the gene transduction plant still exercise its role, which first lines sitosterol content is lowest, soluble starch and soluble protein content of the control did not change significantly, indicating that it can achieve the purpose of the transgenic by The body quality minimal impact, combine 2 in the roots of the target protein detection results of lines 1 molecule detection and quality traits corresponding relationship. Agronomic traits investigated shows that the basic stability of the genetically modified external phenotypic traits, leaf length, leaf width, ear length, between generations strain stability, grain weight, seed rate and plant height intergenerational stability, effective tiller is in lines stable agronomic traits measurement items are displayed stable. The overall performance of the transgenic rice agronomic traits plant height is relatively high, grain weight increase, but lower seed setting rate, indicating that the grain is relatively full, but there are more empty seeds. 5 pollen detected target gene with relatively weak, detection of 3α-HSD protein content is relatively low, indicating that the gene spread through pollen channels with low risk detailed judgment pollen security, can create a diffusion model to further study. Soil isolated bacteria to cholesterol as a carbon source the different strains preliminary identification of E. coli in the three soils. Transgenic rice, the same kind of bacteria isolated from non-transgenic rice with blank soil, secretions of the root of the transgenic rice has no effect on bacterial populations of the same steroid substances as carbon source. Bacterial DNA is not amplified target band, the target protein is not detected, indicating that the 3α-HSD gene is not spontaneous Go homology nearer the risk of bacteria of testosterone Comamonas sp Go plants.

Study on the Hereditary Characteristics and Influences to the Soil Bacteriums of the Rice Offsprings Transferred 3α-HSD Gene

Abstract

Related Dissertations