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In this experiment, rice (Oryza sativa L) as materials, analysis of CK, Cd, Cd Zn, Cd Zn AT (CAT inhibitor, aminotriazole) and Cd Zn DMTU (H202 scavenger, dimethyl thiourea) 5 Daily processing conditions, the rice root growth and gene expression situation. The results show that a single AT, Cd Zn and Cd Zn AT3 kind of treatment are to promote root growth; contrast, single DMTU and Cd Zn DMTU significantly inhibited root growth. In addition to AT treatment, root growth and change and H202 content in basically the same trend, indicating that the H202 on the root growth of a clear regulatory role. Various processes of Evans blue dye shades with H202 and location by showing basically the same rules. Cd stress, add Zn only reduces the accumulation of Cd in roots, but also reduces the H202 in the apical meristem production, meristem region affected by the extent of injury is also reduced. The use of semi-quantitative RT-PCR from a comprehensive analysis of the transcript levels of several treatments at different developmental stages of auxin-related gene family (OsARFs, OsPINs, OsPID, OsIAAs), the core cell cycle gene family (OsCycs, OsCDKs etc.) and OsMAPK gene family expression changes. The results showed that treatment 9 days, Cd Zn and Cd Zn AT treatment process compared to 58 upregulated genes, the most obvious are OsIAA21, OsCycF1; 2 and OsMAPK8; There are 66 genes were down-regulated, such as OsIAA31. Cd Zn and Cd Zn DMTU treatment treatment compared with 49 gene expression increased, as OsIAA18; has decreased expression of 72 genes, such as OsIAA16. Processed 13 days, Cd Zn and Cd Zn AT treatment compared with 68 processing enhancement of gene expression, such as OsARF15; There are 56 genes weakened, as OsKRP1. Cd Zn and Cd Zn DMTU treatment treatment compared with 61 gene expression increased, most notably OsCDK; 1; There are 65 genes decreased, as OsCKL9. These results suggest that, H2O2 and gene expression regulation. The results also suggest that gene expression and developmental stages related, such as OsKRP4 and OsDP1 in dealing with day 9 expression, whereas no expression at 13 days. The experimental results show that in general, Cd stress, adding Zn significantly change the root growth and gene expression, this change is closely related with H202, H202 is to adjust the conditions imply root growth and gene expression signal molecules. H202 signal may be through interaction with auxin and MAPK signaling, the regulation of gene expression and root growth.
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