Dissertation > Excellent graduate degree dissertation topics show

Determination of Endogenous JA in Rice and the Relationship between Its Content Change and Floret Opening

Author: HuXin
Tutor: ZengXiaoChun
School: Jiangxi Agricultural University,
Course: Ecology
Keywords: Rice UPLC-MS/MS Floret opening Endogenous jasmonic acid
CLC: S511
Type: Master's thesis
Year: 2011
Downloads: 145
Quote: 0
Read: Download Dissertation

Abstract


Endogenous jasmonic acid content in rice as test material, the establishment of a stable detection method. And use the method of determination of Rice Floret Opening in endogenous jasmonic acid content of the day, the study the rice endogenous jasmonic acid content changes in the state of nature and the spikelets open relationship, and intends to explore Rice endogenous jasmonic acid is involved in the regulation of floret opening. The specific results are as follows: 1. Seedlings of aluminum, the endogenous jasmonic acid ultra-high performance liquid chromatography - mass spectrometry / mass spectrometry (UPLC-MS/MS) quantitative analysis. Submerged plant tissue samples were extracted with methanol jasmonic acid (jasmonic acid, JA), to extract the columella after solid phase extraction (SPE-NH2) to remove impurities, Dihydrojasmonate (H2JA) as an internal standard, by UPLC-MS / The MS instrument for quantitative analysis. Set 5ng / g, 20ng / g, 100ng / g three spiked levels measured spiked recoveries and its coefficient of variation. The JA and H2JA ES-ionization mode and MRM scan mode, collecting characteristic ions for the the parent ions 209m / z ion 58.7m / z parent ion 211m / z ion 58.7m / z. The results show that the UPLC-MS/MS detection JA showed a good linear relationship in the concentration range of 2ng/ml ~ 100ng/ml, amino column coefficient of variation before and after treatment were: 10.83%, 1.92%, three spiked level of recoveries were 79.16% -93.46%. 2 UPLC-MS/MS Rice Floret Opening day of the the spikelets endogenous jasmonic acid were measured and record field of spikelets open dynamic. The results show: the fertile lines after the rice in a large number of open Ying before changes in endogenous jasmonic acid content with a clear upward trend, floret opening peak, jasmonic acid content began to decline. And fertile lines, sterile line of rice in a large number of spikelets open, endogenous jasmonic acid content did not change significantly, only in relatively concentrated period of floret opening up slightly, after gradually with the reduction in the number of open-Ying reduced. CMS rice spikelets endogenous jasmonic acid content throughout the day little changed compared to fertile lines more stable. Four rice varieties tested, as early as 25, Zhenshan 97A and D38s Jianye endogenous jasmonic acid content spikelets endogenous jasmonic acid content higher than 2-5 times; R402 in both The content is relatively close. The floret opening day, jasmonic acid content of endogenous trend in flag leaves and spikelets consistency. Salicyl hydroxamic acid (SHAM) is the first enzyme in JA biosynthesis inhibitors, 5mmol / L SHAM fertile lines of rice isolated spike dip spike processing. The results showed that: SHAM not open the spikelets showed inhibitory effect, nor did it reduce spikelet endogenous jasmonic acid content, but showed more significant inhibitory effect on the flag leaf endogenous jasmonic acid content, in the treatment of 1 to 5 hours, there is a clear downward trend. Jianye jasmonic acid content changes

Related Dissertations

  1. Study on Rice-Frog and Melon-Frog-fish Ecological Cultivation and Breeding Mode and the Nutrient Balance,S-0
  2. SCXC corporate culture construction,F270
  3. Monitoring of Insecticide Resistance and Resistance Risk Assessment for JS118 in the Rice Stem Borer, Chilo Suppressalis (Walker),S435.112.1
  4. Arthropod Diversity in Non-Crop Habitats at Margins of Paddy Fields of Different Types,S435.112
  5. Detection of the Resistance in Xanthomonas Oryzae PV. Oryzae and X. Oryzae PV. Oryzicola to Two Bactericides and the Assentment of Resistance Risk in Vitro,S435.111.4
  6. Study on Application of Red Yeast Rice in Fermented Sausage,TS251.65
  7. The Research on Improvement of Rice Noodles Quality,TS213.3
  8. Studies on the Properties of Starch from Germinated Polished-rice-with-germ,TS235.1
  9. The Research on the Comprehensive Modification Technology of Rice Protein Enzymatichydrolysis and Graft Copolymerization,TS201.2
  10. The Rice OsNAR2.1 Participates the Regulating Root Growth by Nitatre,S511
  11. Research on Feed Safety of Transgenic Rice in Broiler,S831.5
  12. Soil Eoclogical Risk Evaluation of Transgenic Bt Rice Through Nematode Community Analysis,S154.1
  13. Genetic Analysis and Molecular Mapping of QTL for Rice Black-Streaked Dwarf Disease Resistance in Rice,S511
  14. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  15. Concentrations of Lipids in Grains of Japonica Rice: In Response to Nitrogen Fertilizer and in Relation to Physicochemical Properties of Rice Flour,S511.22
  16. Genetic Segregation Analysis and QTL Mapping as Well as Association Analysis for Panicle Angle Trait in Japonica Rice (Oryza Sativa L.),S511.22
  17. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  18. Characteristics of Over-Expression for Nitrate Transporter Genes OsNRT1.2 and OsNRT1.5 in Rice,S511
  19. Research on Influnce of Silicon and Sulfur on Arsenic Uptake and Accumulation of Rice (Oryza Sativa L.),S511
  20. The Effectr of Lactic Acid Bacteria and Rice Bran on Dynamic Change of Rice Straw Silage Fermentation,S816.53
  21. Bioinformatics Study of Motifs Within 3’UTR Region of Stress Response Genes and Functional Related Mirnas in Rice,Q943.2

CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
© 2012 www.DissertationTopic.Net  Mobile