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Functional Analysis of Rice Phytochrome A in Arabidopsis

Author: ChenChunFeng
Tutor: WangYaQin
School: South China University of Technology
Course: Biochemistry and Molecular Biology
Keywords: Arabidopsis OsPHYA Seed germination Hypocotyl Chlorophyll synthesis EOD FR response
CLC: S511
Type: Master's thesis
Year: 2011
Downloads: 42
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Abstract


As an important environmental signal light, has a broad regulatory role in the growth and development of plants. Higher plants in a constant process of evolution formed a complete network of light signaling, by photoreceptors perceive light changes in the environment, and continue to adapt to the environment, to promote their own growth and development. These photoreceptors, the photosensitizing dye is by far the most clearly studied photoreceptors, it plays an important role in the process of the plant photomorphogenesis. Phytochrome A light receptors in higher plants regulate far-red light induced reaction. The papers by constructing rice phytochrome A overexpression carrier expression in transgenic plants with WT / OsPHYA and complementary transformed plants phyA-211/OsPHYA, through the conversion of the two plants, wild-type and phyA mutant phenotype analysis, research rice PHYA function in Arabidopsis, the main findings are as follows: (1) transfer of transgenic plants WT / OsPHYA and phyA-211/OsPHYA homozygotes obtained: Basta resistance screening,, get the WT / OsPHYA and the phyA-211/OsPHYA, positive plants in the T3 generation homozygote; detected by RT-PCR and Western Blot OsPHYA gene expression could in transgenic plants with WT / OsPHYA and complementarity of the conversion plants phyA-211/OsPHYA in normal expression. (2) under different light conditions, OsPHYA Seed Germination: wild-type WT and mutant body phyA-211 the the WT / OsPHYA and phyA-211/OsPHYA homozygotes seeds were placed in VLFR light conditions, shade reaction light conditions Determination of germination rate, results showed that the seed germination rate the the WT / OsPHYA and phyA-211/OsPHYA can reach more than 90%, significantly higher than the mutant phyA-211 Description exogenous OsPHYA and low R / FR pulse conditions be able to play a role in promoting seed germination. (3) different types of continuous light, of OsPHYA hypocotyl elongation: Seedlings of four kinds of materials in different under continuous illumination after culture hypocotyl measurement. The results showed that continuous far-red light irradiation the, phyA-211/OsPHYA hypocotyl length less than phyA-211, hypocotyl inhibition produced in the context of exogenous OsPHYA including source AtPHYA missing part to make up for the AtPHYA function; Similarly, in the WT / OsPHYA also appeared the same inhibitory effect, the hypocotyl length less than the wild-type, that the premise of the existence of endogenous AtPHYA exogenous OsPHYA also possible to suppress a hypocotyl extension. (4) role of OsPHYA albino go yellow in: culture in the light of the four materials albino to green ratio and chlorophyll content. The results show that the two transgenic plants the WT / OsPHYA and phyA-211/OsPHYA under various processing conditions and turn green ratio of the wild-type WT is basically close description the exogenous OsPHYA role instead the source AtPHYA mutant, albino seedlings to green play a role. The the WT / OsPHYA chloroplast content under various processing conditions are the highest mutant phyA-211 phyA-211/OsPHYA chloroplast content compared to the latter is significantly higher to illustrate outside source OsPHYA in the process of chlorophyll synthesis. (5) OsPHYA EOD FR response: four materials on EOD FR light conditions measured hypocotyls, petioles and flowering time, the results showed that the wild-type WT and phyA-211/OsPHYA compared under the hypocotyl length close to the petiole length difference larger flowering time is close to, the the exogenous OsPHYA can feel EOD FR light stimulation and inhibition of hypocotyl elongation, promote early flowering of plants. But in the the PHYB context, for the inhibition of the petiole is not obvious.

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