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Research wheat import to the general effect of the EU and its quality goat gene Grass Valley

Author: SunBin
Tutor: RenZhengLong
School: University of Electronic Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Ae. biuncialis HMW glutenin SDS-PAGE cytogenetic analysis bread-making quality
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 28
Quote: 0
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Abstract


Wheat is the most important grain crop in the world. During modern crop breeding, common wheat has a very narrow genetic variation, thus its resistance ability to diseases, pests and extreme environmental factors is limited, in the mean time the yield and quality have been restricted. The genus Aegilops, which is the closest relative to wheat and carries numerous genes of agronomic interest, can be incorporated into the wheat genome. Aegilops biuncialis （2n = 4x = 28, UbUbMbMb） is a tetraploid wild relative of wheat belonging to Ae. Umbellulata. It has agronomically interesting traits, such as resistance drought, drought stress tolerance, salt stress tolerance, disease resistance, strong tillering ability and high percentage of ear-bearing tiller.Therefore, Ae.biuncialis plays an important role not only in the evolution of cultivated wheat but also the processes of improving the genetic variation of common wheat. Ae.biuncialis carries two unique HMW glutenins subunits named as Glu-Aeb1 and Glu-Aeb2 which were incorporated into the wheat. In order to study the effect on wheat quality, we carried out some research concerning about the transfer frequency of HMW glutenin, cytogenetic analysis and bread-making quality. The results are reported as follows:1. 21 Ae. biuncialis and common wheat hybrid progenie（sBC2F6）,along with 15 hybrids（F0）of BC2F5 and common wheat hybrid progenies, were studied using SDS-PAGE. The results showed that all the materials are in possession of Ae. biuncialis HMW glutenin subunits. The transfer frequencies of Glu-Aeb1 in BC2F6 and F0 are 59.52% and 20.94%, respectively. While the transfer frequencies of Glu-Aeb2 in BC2F6 and F0 are 40.48% and 22.96%, respectively.We obtained two materials which are in possession of Glu-Aeb1 and Glu-Aeb2, respectively.2.C-banding and in situ hybridization analysis were used to study the materials which are in possession of Ae. biuncialis HMW glutenin subunits. Line 12-5-1 has 42 chromosomes based on the chromosomal counting of cell of the root tips. C-banding indicates that chromosome 1B is absent. SDS-PAGE also conformed that the absence of 7+8 subunits which were encoded by Glu-B1. Comparing with standard chromosome bands we are able to identify the substitute chromosomes 1M from Ae. biuncialis. FISH was carried out using pAT71 as probe hybrid on 12-5-1, and the results show that there are six hybridization signals on different chromosome. Line 12-5-2 has 44 chromosomes based on the chromosomal counting of cell of the root tips including2 chromosomes with trait bands of Ae. biuncialis chromosome 1U, preliminarily determined 12-5-2 as 1U disomic chromosome additions.3. We had determined protein content in materials 12-5-1 and 12-5-2, Zeleny, content of wet gluten and water content, and analyzed amplitude of variation, variation coefficient and standard deviation. From that incorporated Glu-Aeb1 and Glu-Aeb2 has positive effect on wheat somehow.In order to conform the interaction, relationship between the quality traits should be analyzed in the further research.

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