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Ramie unique as our traditional textile materials , degumming ramie spinning a necessary process . Traditional chemical methods unglued heavy pollution , high energy consumption , the biological degumming mild reaction conditions , light pollution , low energy consumption , cleaner production technology is urgently needed by the textile industry . However, due to the lack of biological retting bacteria degumming degumming conditions are too harsh, and limit the industrial applications of bio- degumming technology , need to be screened to complement the new retting bacteria or try new microorganisms using . To clarify the degumming Microbial Resources available in natural or artificial unglued environment , as well as the natural microbial retting way , is the development and utilization of new retting bacteria or indirect use of the premise and foundation of degumming microbial genetic resources useful . The from different retting or rotting hemp habitat screening to the artificial domestication get mixed the degumming flora RAMCD407 . Using PCR -DGGE ( polymerase chain reaction and denaturing gradient gel electrophoresis , PCR -DGGE ) technology , combined with traditional plate isolated and cultured microflora analysis , biological degumming of ramie commensal flora . The experimental basal medium ramie powder medium , the ramie fermentation material medium of strains were isolated and purified , respectively, 12 , 10 , 10 pure cultures of bacteria . Biochemistry and molecular identification results with six genus homology higher were tufted Aeromonas spp ( Comamonas sp . ) , Pseudomonas aeruginosa (Pseudomonas aeruginosa), Serratia (SerratiaBizio) , Enterococcus (Enterococcus), Bacillus subtilis (Bacillus subtilis), Bacillus macerans (Bacillus macerans). PCR-DGGE analysis of flora community structure , exploration and optimization of experimental system . The experiment eventually using the freeze-thaw - proteinase K-SDS method to extract strain total DNA . The the DGGE system is optimized denaturant gradient of 30 % to 60 % , the gel concentration of 8.0% , temperature 60 ° C , 120V electrophoresis approximately 9.5h . The optimal electrophoresis conditions , the select separation of a pure culture of strain and mixed flora , establish their DGGE fingerprint strip recovery sequencing , provide the basis for further degumming studies .
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