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Study on Changes Chemical Substances of Diapause Induction and Breaking Diapause in Trichogramma Dendrolimi Matsumura

Author: WangSheng
Tutor: SunGuangZhi
School: Jilin Agricultural University
Course: Agricultural Entomology and Pest Control
Keywords: Trichogramma dendrolimi diapause terminating diapause physiology and biochemistry
CLC: S476.3
Type: Master's thesis
Year: 2011
Downloads: 62
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Abstract

The study of diapause on Trichogramma spp. may be of important significance in mass rearing and utilization. This research was sumed up the condition in diapause inducing period and breaking diapause period which based on the predecessor findings at temperature, maternal photoperiod, ages etc. the Chemical Substances of diapause induction and breaking diapause in Trichogramma dendrolimi was test,The purpose that is to realizeon the T. dendrolimi physiology and biochemistry changes in this long period.This study may be a good foundation for the application of diapause in Trichogramma mass rearing, utilization and other relative researches.Inspected the inducing diapause rate at inducing diapause period at 11℃, in 30 days and breaking diapause period at 3℃, in 120 days in T. dendrolimi respectively. The results showed that it could induce diapause inducing diapause in T.dendrolimi at 11℃, The proportion of diapause rate reached the highest (98.17%) when in 30 days during inducing diapause period, then continue to inducing breaking diapause period at 3℃,the inducing diapause rate decreased with the time, in 90 days the inducing diapause rate was 13.25%,in 100 days was only 7.2%.and the whole process between inducing diapause and inducing diapause period was most significant.Tested the content of total carbohydrate in different disposal period at non-diapause、inducing diapause and breaking diapause. The results showed that non-diapause at 25℃the content of total carbohydrate was increase before 4 days,then decrease after 3 days, Total carbohydrate show on the most vaule 5.38 ug/mg at the 8th day,and the difference between them was significant.In induce diapause period at 11℃, the content of total carbohydrate was increase with the induce time,at 30d the vaule was 9.09μg/mg; In induce breaking diapause period at 3℃, the content of total carbohydrate was continue increase,at 110 d was up to the most vaule (11.05μg/mg),at 150 d was decrease to the law vauleth (7.71μg/mg), and the whole process between inducing diapause and inducing diapause period was most significant.Tested the content of Protein in different disposal period at non-diapause、inducing diapause and breaking diapause. The results showed that non-diapause at 25℃the content of Protein was decrease before 2 days, increase after 2 days,then decrease before 2 days fianally. the content of Protein was at 5th 201.82μg/mg reduce to 43.46μg/mg at 11th,the non-diapause process was most significant. In induce diapause period at 11℃, the content of Protein was continue decrease with the induce time,the vaule was 224.98μg/mg at 15 d reduce to 139.33μg/mg at 30 d, the diapause process was most significant; In induce breaking diapause period at 3℃, Protein content was maintained consistently a level of 99.77～102.34μg/mg during inducing breaking diapause. and the difference between them was not significant,Tested the content of Protein in different disposal period at non-diapause、inducing diapause and breaking diapause. The results showed that non-diapause at 25℃the content of Glycerine was increase before 3 days, then decrease after 3 days fianally. the content of Glycerine was the up to the most vaule at 8th 29.37μg/mg,the non-diapause process was most significant. In induce diapause period at 11℃, the content of Glycerine was continue increase with the induce time,the vaule was 54.55μg/mg at 30 d, the induce diapause process was most significant; In induce breaking diapause period at 3℃, the content of Glycerine was at 60 d 78.39μg/mg reduce to 47.04μg/mg at 150 d.and the difference between them was most significant.Tested the content of SOD、POD、CAT in different disposal period at non-diapause、inducing diapause and breaking diapause. The results showed that non-diapause at 25℃the content of SOD was increase before 2 days, then decrease after 4 days fianally.at 7th show on the most vaule 315.37 U/mgprot,athe SOD vaule was 231.76 U/mgprot at 10th added to 270.75 U/mgprot at 11th. the non-diapause process was significant. In induce diapause period at 11℃and induce breaking diapause at 3℃, the content of SOD was first increase then decrease as the whole. The lowest content of SOD was 174.23 U/mgprot at 20 d,then increase to 449.81 U/mgprot at 90 d.as a result,the content of SOD was reduce to 227.32 U/mgprot.the content of SOD at inducing period was higher then the non-diapause. the whole induce diapause process was most significant. The non-diapause at 25℃the content of POD showed on"W"figure,the most lawest vaule was 0.49 U/mgprot at 9th and the most highest was 1.04 U/mgprot 11th, the non-diapause process was significant. In induce diapause period at 11℃and induce breaking diapause at 3℃, the content of POD showed on "M"figure.the content of POD was 0.53 U/mgprot at 15 d up to 1.82 U/mgprot at 90 d.though the content of POD was only 0.96 U/mgprot at 150 d. the conten of POD at inducing period was higher then the non-diapause. The whole induce diapause process was most significant.The non-diapause at 25℃the content of CAT was increase before 2 days, then decrease after 4 days fianally.the content of CAT show on the vaule 5.24 U/mgprot at 5th increase to the most vaule 12.52 U/mgprot at 7th.then CAT vaule was continue to reduce until the most lowest 4.42 U/mgprot at 11th. the non-diapause process was most significant. In induce diapause period at 11℃and induce breaking diapause at 3℃, the content of POD showed on"M"figure,the content of CAT was increase at 15 d from2.93 U/mgprot to 20.99 U/mgprot the most vaule at 60 d.then reduce to the lowest content of CAT was 8.97 U/mgprot at 150 d,the content of CAT at inducing period was higher then the non-diapause. The whole induce diapause process was most significant.According to the SDS-PAGE include non-diapause、inducing diapause period inducing breaking diapause period. The results of electrophoresis diagrams showed that expressive on non-diapause was more changes then inducing diapause,and inducing diapause period with inducing breaking diapause was most signinicant.

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