Dissertation > Excellent graduate degree dissertation topics show

Ester-type Catechins on HeLa Cell Proliferation and Its Agents (Vaginal Suppository Carrier)Quality Standards

Author: LiuJinWen
Tutor: LiHongYu
School: Liaoning Medical
Course: Pharmaceutical Analysis
Keywords: Ester type catechins Vaginal suppository carrier Quality standards HeLa cells
CLC: R943
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
Read: Download Dissertation

Abstract


Objective worldwide per year, nearly 500,000 new cases of cervical cancer, the number of deaths due to cervical cancer up to 27 million people. Large number of epidemiological and molecular biology research data shows that human papillomavirus (HPV) and cervical precancerous lesions are closely related, ester-type catechins have anti-viral, anti-cancer effect. The purpose of this study is to investigate the ester type catechins on human cervical carcinoma HeLa cell proliferation, and work out its preparations acetate ester type catechin catechins vaginal suppository vehicle quality standards, to achieve the purpose of prevention and treatment of cervical cancer. Methods This study used MTT assay ester type catechins different concentrations and different time of HeLa cells in vitro inhibition; fluorescence staining using different concentrations of ester-type catechins morphological changes in HeLa cells. This study used qualitative identification by TLC ester type catechins; chemical identification method using auxiliary qualitative identification ester type catechins; using high performance liquid chromatography assay for quantification of ester-type catechins; using thioacetamide France ester type checking catechin lead levels; using ancient Cai type method check ester type catechins arsenic; drying method using ester-type catechins check the moisture content. In this study, by HPLC identification and quantitative determination of ester-type catechins vaginal suppository carrier; chemical identification method using qualitative identification of ester-type catechins assisted vaginal suppository carrier; using disintegration test method for detecting vaginal ester type catechins Carrier tied disintegration test. The results of this study demonstrate ester type catechin on the growth of HeLa cells was significantly inhibited, and with drug concentration and time increases gradually increased, showing the time - dose dependent. And ester-type catechins from chemical identification, TLC impurities, determination of other aspects of APIs to develop quality standards. From the chemical identification, suppositories, determination to develop other aspects of the ester-type catechins vaginal suppository carrier quality standards. Conclusion 1, ester-type catechins on human cervical carcinoma HeLa cells was strongly inhibited, in a certain range in a dose and time dependent, preliminary evidence of ester-type catechins have a certain role in the prevention and treatment of cervical cancer, prevention and treatment of cervical cancer can be used as medicine. 2, the establishment of a more complete its preparations ester type catechin quality standards, this study used qualitative identification method is specific, simple and convenient; determination method is accurate and reliable; and on bulk drugs and suppositories may exist the impurities affect the quality of the limited. Effectively guarantee the ester and ester type catechin EGCG vaginal suppository quality control carrier.

Related Dissertations

  1. Exploratory Studies on the Effective Fractions of Tianshu Capsule,R283
  2. Studies on the Quality Standard of Brassica Rapa L.,R284.1
  3. Preparation of Compound wind particles and preparation of quality standard,TQ461
  4. Forsythia concocted and forsythiaside A Study on Separation and Purification,TQ461
  5. Study on the Fu Ante Oral-nano Granular Formulation,R286.0
  6. Study on Screening and Inhibition on Human Cervical Cancer HeLa Cells of Active Components from Walnut Pericarp,R284
  7. The Study on the Content Determint, Fingerprint and Dissolution of ShenFuShu Granules,R286.0
  8. The Eukaryotic Expression of Foot-and-mouth Disease Virus Structural Protein VP4 Gene,S852.65
  9. Study on Lab-scale Process Development and Manufacture of Atipamezole HCI,TQ463.53
  10. Trauma wishful gel preparation process , quality standards and preliminary stability study,TQ461
  11. Study on the Preparation and Quality Standard of Bushen Jiangu Tablets,TQ461
  12. PAC-1 hydrochloride quality standard APIs,R927
  13. 1.Proteome Comparative Analysis of Low-Grade Human Brain Astrocytomas and AdjacentNormal Gliomas 2.Proteome Comparative Analysis of Hela Cells Transfected with TRIM45 Gene,R739.41
  14. Effects of Shikonin and Other Extractions on the Growth of in Vitro Culture HeLa Cell,R285
  15. Primary Study of Antitumor Effect and Mechanism of Sesamum Inducum L.flower Extract in Vitro,R285.5
  16. Malt treatment of hyperprolactinemia and heather vine quality standard of,R259
  17. Study of the Effect of Textile Chemicals on the Growth of Ectogenetic Cells,TS195.1
  18. Research on the Preparation and Quality Standard of Chaihuang Soft Capsule,TQ461
  19. Effect of Gossypol and Gossypol-based Combination DDP on the Proliferation and Apoptosis of Cervical Carcinoma Hela Cells,R737.33
  20. Compound muskrat pills molding process and quality standard,R286
  21. Study on the Preparation and Quality Standards of Forsythia and Amoxicillin Powder,R943

CLC: > Medicine, health > Pharmacy > Pharmacy > Pharmaceutics
© 2012 www.DissertationTopic.Net  Mobile