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Observations on Biological Characteristics of the Human Gastrointestinal Stromal Tumor Cell Line (GIST-H1)

Author: LiuSheng
Tutor: LiaoGuoQing
School: Central South University
Course: General Surgery
Keywords: gastrointestinal stromal tumors cell line gene sequencing biological characteristics ability of invasion
CLC: R735
Type: Master's thesis
Year: 2011
Downloads: 33
Quote: 0
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Abstract


Objective:To further observe the biological characteristics of human gastrointestinal stromal tumor cell line (GIST-H1).Methods:The morphological observation of the cell climbing slice stained by hematoxylin-eosin and gene sequencing for the cells passing from the stromal tumor GIST-HI cell line, gene sequencing for the original organization and the immunohistochemistry test for the tumorigenicity in nude mice were performed to further identify their biological characteristics; and the invasion and metastaticabilityof the GIST-H1 cells was investigated in vitro by the Transwell invasion assay.Results:1. The cell climbing slices stained by hematoxylin-eosin were in typical spindle shape with light microscope.The nucleocyto-plasmic ratio was enlarged.2. The Transwell invasion assay indicated that the mean cell number of the 15 high power field for the GIST-H1 cell line was 44 while that of the normal gastric mucosa for the GES-1 cell line was 0.3. None of the mutations were found in exons 9,11,13 and 17 of the C-kit gene, nor were exons 12,14 and 18 of PDGFRA gene. There is a insertion mutation in the upstream of exon 18.4.The GIST-H1cell line and original organization are homologous.5. The immunohistochemistry test for the tumorigenicity in nude mice showed the CD117 was weak positive. Conclusions:1. GIST-H1 cell line has typical biological characteristics of the gastrointestinal stromal tumors.2. GIST-H1 cell line might belong to the wild type for its bearing no common site mutation.3. The cells splitting from GIST-H1 cell line possess an obvious capability of invasion.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer
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