Dissertation > Excellent graduate degree dissertation topics show

The Changes of β-defensin-3 Protein Level in Bronchoalveolar Lavage Fluid of Children with Mycoplasma Pneumoniae Pneumonia

Author: LiJuan
Tutor: ZhongLiLi
School: Hunan Normal University
Course: Internal Medicine
Keywords: Mycoplasma pneumonia humanβ-defensin 3 bronchoalveolar lavage The copies of MP gene children
CLC: R725.6
Type: Master's thesis
Year: 2011
Downloads: 51
Quote: 0
Read: Download Dissertation

Abstract


Objects:①To observe human beta-defensin-3 (hBD-3) protein level in children of Mycoplasma pneumoniae pneumonia (MPP);②To disscusse the relationship between hBD-3 protein level in BALF and the copies of MP 16S rRNA gene in BALF, the values of C-reaction protein (CRP) respectively in serum of MPP children;③To observe differential hBD-3 protein levels in MPP children between before and after therapy, in order to understand the role of hBD-3 in MP infection.Methods:1. Object and grouping①The 63 patients with pneumonia in our department during July 2009 to November 2010 were enrolled and the paired serum of each patient were collected. The specific antibody of MP was detected by partical agglutinate method. It was taken as the diagnostic criteria of MP infection that the titer of specific antibody of MP in the second serum increasing exceed 4 times than that in the first serum.②According to the diagnostic criteria,63 patients were divided into two groups, MPP group (n=36) and nMPP group (n=27).③According to the diagnostic criteria of RMPP, MPP group (n=24) were divided into RMPP group (n=22) and common MPP group (n=12). All the enrolled patients were given flexible fiberoptic bronchoscopy and their BALF were collected.④In MPP group, paired BALF of 10 patients were kept before and after 7-14 days therapy.⑤Meanwhile,12 re-examinated cases who were given the operation of removing airway foreign bodies four weeks before were taken as control group and their paired serum and BALF were collected.2. The BALF were collected by flexible fiberoptic bronchoscopy, and then centrifuged. And 10 patients in MPP group were given flexible fiberoptic bronchoscopy twice and paired BALF were reserved.3. hBD-3 protein level in supernatant of BALF was detected by ELISA while total protein level in supernatant was detected by Biuret method. FQ-PCR was used to detect MP 16SrRNA gene in deposit of BALF.4. The relationship between hBD-3 protein level and the copies of MP 16S rRNA gene, the values of C-reaction protein (CRP) respectively of MPP children were observed; Differential hBD-3 protein levels and copies of MP 16SrRNA gene between before and after 7-14 days therapy in MPP children were compared.Results:1. hBD-3 level in BALF of children with MPP, non-MPP and that of control group was 227.99+37.25 pg/mg,232.17±17.40pg/mg, 340.98±19.6 pg/mg, respectively. The hBD-3 level in MPP group and non-MPP group was significantly lower than that of control group (t=15.31 and 10.11 respectively, P<0.01). Comparied with non-MPP group, the level of hBD-3 in MPP group has no significant difference (t=0.49 P>0.05).2. The copies of MP 16S rRNA gene in BALF of 36 MPP patients were 3.80×104-4.70×.108copies/ml, log10=5.99±0.98. In non-MPP and control group, the results were negative.3. In MPP group, the hBD-3 levels were negatively correlated with the copies of MP 16S rRNA gene. (γ=-0.73, P<0.05).4. In MPP group, hBD -3 levels were negatively correlated with CRP values. (γ=-0.56, P<0.05). The copies of MP 16S rRNA gene in BALF of MPP patients were highly correlated with CRP values. (γ= 0.81, P<0.05).5. In RMPP group, the hBD-3 level (226.87±36.84 pg/mg) was significantly lower than that of common MPP group (237.45±41.5 pg/mg) (t=17.78, P<0.01). The copies of MP 16S rRNA gene in BALF of RMPP were 3.80×104-4.70×108, log10=6.03±0.97. For common MPP group, the copies of MP 16S rRNA gene in BALF were 4.10×104-1.30×108, log10=5.86±1.05. The result showed that the copies of MP 16S rRNA gene in common MPP group were significantly higher than those of RMPP group (t=15.97, P<0.01).6. The hBD-3 level in BALF of 10 MPP patients before and after therapy was 246.94±22.54 pg/mg,274.10±25.02 pg/mg, respectively. The latter was significantly higher (t=5.873 P<0.01). The copies of MP 16S rRNA gene in BALF of 10 MPP patients before therapy were 1.30×105-8.70×105 copies/ml, log10 5.51±0.25, while after therapy, the copies of MP 16S rRNA gene were 3.4×103 2.1×104 copies/ml, log10 3.9±0.25. The latter was significantly lower (t=7.90 P<0.01)Conclusion:The hBD-3 levels in BALF of MPP patients were significantly lower than those of control group and the descent extent of hBD-3 were related with the copies of MP gene and severity of pathogenetic condition. The hBD -3 levels can rise up after treatment. Those suggestted that the descent of hBD -3 levels in BALF of MPP patients may be associated with airway epithelial cell damage, and may contribute to the pathogenesis of RMPP.

Related Dissertations

  1. The Research of Children’s Medical Environment Based on New Medical Mode,TU246.1
  2. The Influence of Reputation on Preschool Children’s Transgression Behaviors,B844.1
  3. Social exclusion of migrant children in the Perspective of the New Exploration of compulsory education,G521
  4. Professor Kong Guangyi academic thought Qing Xuan main Treating children cold cough,clinical research and clinical experience,R249.2
  5. Professor Wang Yinglin academic thought and clinical experience and treatment of the palate and features of drug research in the original look of the children cold fever,R249.2
  6. Space environment of rural children left behind boarding schools,TU244.2
  7. Research on Present Situation and Influence Factors about Young Chrendren’s Tennis in Xi’an,G845
  8. Research on Influences and Defense Mechanism of Education in Cultivation for Children Left Behind in Village by Vulgar Culture,G632.4
  9. Retrospective Study of Asthmatic Children Received Inhaled Corticosteroid Therapy,R725.6
  10. Junior high school children, mothers induced anger and Related Issues,B842.6
  11. Clinical analysis of 268 cases of premature,R714.2
  12. Art Education for Single-Parent Families Are Psychological Barriers Children the Importance of Exploring Research,G623.75
  13. The Case Study of the School Factors on the Academic Achievement of the Left-behind Children,G521
  14. Practical Strategies on the Education of Children’s Rhythms in Primary School,G623.2
  15. The Application of Game Teaching Method in the Chinese Teaching to Korean Children,H195
  16. A Research on Gender Implicit Stereotypes about Different Types of Activities,G645.5
  17. Village Children’s Socialization under the Background of Rural Culture Changing,C913.5
  18. Socialization Deviation of the Floating Children During the Period of Social Transformantion,B849
  19. The Study of Myocardial Injury on Herpangina in Children Who Was Acut,R766.1
  20. Research on the Teaching Reform of the Vocal Music of the Preschool Education,J616
  21. Human cytomegalovirus infection and childhood idiopathic thrombocytopenic purpura clinical analysis,R725.5

CLC: > Medicine, health > Pediatrics > Children within the science > Department of pediatric respiratory and chest diseases
© 2012 www.DissertationTopic.Net  Mobile