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Morphological Observation of the Reversal of Ventricular Hypertrophy in Rat by Loosening Abdominal Aortic Banding

Author: ZuoYangTian
Tutor: FengYaoGuang
School: Nanhua University
Course: Surgery
Keywords: Left ventricular hypertrophy Morphology
CLC: R654.3
Type: Master's thesis
Year: 2011
Downloads: 16
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Abstract


[Objective] To understand the success by lifting the rat abdominal aortic coarctation method reverse left ventricular hypertrophy, and compare reverse rat heart and left ventricular hypertrophy of the heart and the morphology of normal rat heart, collagen volume fraction and apoptotic index difference. [Methods] SD rats 75, male and female, were randomly divided into control group (N group, n = 15) and left ventricular hypertrophy (VH group, n = 60), left ventricular hypertrophy (VH group) in experiment before the first surgery by constricting the abdominal aorta establishment of left ventricular hypertrophy. After five weeks of the experiment to confirm the modeling success, left ventricular hypertrophy (VH group) were randomly divided into coarctation group (VHS group, n = 20) and lift constriction group (VHJ group, n = 26). Relieve narrowing the group (VHJ group) rats underwent a second surgery to remove the the abdominal aortic original narrow. Echocardiography, biochemical, conventional pathology and quantitative pathology of a variety of technical methods, all the rats in the experiment 6,9 weeks, morphological observation and morphological quantitative analysis. [Results] The experimental rats after the first surgery, 11 died, three died in the second surgery, 18.67% of the total mortality. 5 weeks of the experiment, the interventricular septum end-diastolic thickness of left ventricular hypertrophy (VH group) rats compared with blank control group (N group) significantly thicker (p lt; 0.01), the thickening of the left ventricular posterior wall end-diastolic thickness (p lt; 0.05); heart mass index (HMI) and left ventricular mass index (LVMI) increased significantly (p lt; 0.01). Observe the morphological changes: blank control group (N): see the organizational structure of the normal rat heart, myocardial morphologically normal, neat, intracellular cardiac muscle fibers arranged in neat rows. Uniformity of nuclear size, center or home side, rich sarcoplasmic muscle fibers ARTS clear. Constriction group (VHS) rat: myocardial cells increases, thickening, nuclear stained increases, disordered arrangement of the cells within the myocardial fibers, thickening of the muscle fibers became longer and more branching. The Masson staining: collagen fibers green, to see collagen fiber distribution in small blood vessels and cardiomyocytes, the collagen hyperplasia than unsqueezed group significantly. The myocardial tissue TUNEL () cells than unsqueezed group. The solution shrink group (VHJ). Rats: myocardial cells increased thickening than narrowing the group to alleviate intracellular the myocardial fibers row more regular, no muscle fiber thickening, variable length, the branch, the nucleus than the rule, the cytoplasm stained uniform. Masson staining collagen fibers than normal myocardial tissue still visible TUNEL () cells, and more than normal. The VHS group and VHJ of group six weeks, nine weeks apoptosis index (APOI) compared with the control group (N) was significantly higher (P lt; 0.01), the VHS group, 6 weeks, the nine weeks apoptosis index (APOI) than VHJ group significantly higher (P lt; 0.01). VHS group and VHJ of group of six weeks, nine weeks of myocardial collagen volume fraction (CVF%) compared with the control group (N) was significantly higher (P lt; 0.01), the VHS group, 6 weeks, compared with nine weeks of myocardial collagen volume fraction (CVF The%) VHJ Group significantly increased (P lt; 0.01) Conclusion】 by lifting the rat abdominal aortic coarctation can partially reverse left ventricular hypertrophy reversal rat cardiac morphology, myocardial collagen volume fraction (CVF%), apoptotic index (APOI) than narrowing the undischarged group a significant improvement, but there are still differences in normal rats.

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CLC: > Medicine, health > Surgery > Of surgery > Cardiovascular and lymphatic system surgery > Vascular
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