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Objective: aromatic resuscitation drugs traditional medicinal knowledge and efficacy of modern research, parallel study of the drug (musk, borneol, benzoin and storax incense) to normal and the blood-brain barrier function and brain of the mouse model of ischemia-reperfusion injury in rats the impact of the initial clarify the medicines resuscitation the refreshing efficacy and pharmacological effect against cerebral ischemia connotation, and thus revealed the biological characterization of the drug-like part of the medicinal and also seek common substances of the blood-brain barrier for the excavation of aromatic resuscitation drugs experimental basis. Methods: ① formamide soak fluorescence method for the determination of normal mice and cerebral ischemia and reperfusion injury in mice brain tissue content of Evans Blue (Evans blue, EB) to evaluate the four taste aromatic resuscitation drugs (borneol, musk, Su Hong, benzoin) and its extract parts of the blood-brain barrier (blood brain barrier, BBB) permeability. The ② line intraluminal focal cerebral ischemia reperfusion injury in rat models, respectively, before surgery, reperfusion 4h, 10h, 22h after anal moderate body weight of the rats was measured, before surgery, and re- take ischemic side of the brain to measure its moisture content measured after reperfusion 22h ECG recording the rats from anesthesia to awakening time to observe the rats in each group after reperfusion 4h neurological signs change at reperfusion 22h. ③ colorimetric determination of cerebral ischemia and reperfusion injury in rat serum with ischemic brain tissue superoxide dismutase (SOD) activity, malondialdehyde (MDA), and serum nitric oxide (NO), nitric oxide synthase (NOS), and ischemic brain tissue Ca2 content, Na-K-ATPase, Ca2-Mg2-ATPase (?) (?) sex. Double-antibody sandwich enzyme-linked immune adsorption method (ELISA) Determination of serum tumor necrosis factor α (of TNF-a) level, ischemic side of the brain tissue hormone-1β (IL-1β), interleukin White mediated prime -6 (IL-6) of content . ④ transmission electron microscopy cerebral ischemia-reperfusion injury ischemic hemisphere frontoparietal cortex blood-brain barrier ultrastructure ELISA assay ischemic brain tissue of vascular endothelial growth factor (VEGF) and matrix metalloproteinase - 9 (MMP-9) of the content. The ⑤ line intraluminal focal cerebral ischemia-reperfusion injury in rat model, the colorimetric determination of cerebral ischemia and reperfusion injury in rat cardiac tissue MDA content and SOD, Na-K-ATPase, Ca2-Mg2-ATPase activity . Results: (1) the borneol drug (0.2g/kg), musk-wide drug and musk petroleum ether extract the site (0.0666g/kg), Styrax drug Styrax petroleum ether and ether extract fractions (1.332g/kg), benzoin full drug (1g/kg) was significant elevated normal mouse brain tissue EB content; the borneol drugs (0.2g/kg), musk-wide drug and musk petroleum ether extract fractions (0.0666g/kg) can significantly reduce brain missing blood reperfusion injury model mice brain tissue EB content. ② borneol (0.2g/kg) can significantly inhibit cerebral ischemia in rats 2h reperfusion 4h, 10h, 22h abnormally elevated body temperature the musk (0.0666g/kg) and storax (1.332g/kg), only in reperfusion after 4h, significantly inhibited the increase in body temperature, benzoin (1.0g/kg) is not obvious. Borneol in rats reperfusion can significantly inhibit weight loss 10h when reperfusion 4h and 22h inhibit weight loss trend; and musk, Styrax benzoin insignificant. Rats in each group postoperative heart rate showed varying degrees of slowing the borneol rats postoperative heart rate slowed down, rat heart rate changes in the value of the rest of the drug group was not significantly different. Borneol rats postoperative recovery time was significantly longer, musk rats postoperative recovery time was significantly shorter, but not statistically significant. Four Kinds of drugs on the rat model of behavior scores were necessarily reduce the trend, no statistically significant difference. Musk, borneol could significantly reduce the water content of cerebral ischemia-reperfusion injury in rat brain. ③ musk, borneol significantly improved rat ischemic brain tissue Na-K-ATPase Ca2-Mg2-ATPase, SOD vitality, at the same time can significantly increase serum SOD activity and decrease MDA, NO, NOS, TNF-α and missing blood side of the brain tissue Ca2 of IL-1β, of IL-6 content; Styrax significantly improve ischemic brain tissue Na-K-ATPase vitality, reduce the MDA content of NO, of TNF-α; benzoin significantly with sex to reduce serum TNF-α content. The ④ model and vehicle group ischemic hemisphere frontoparietal cortex area of ??capillary endothelial cells, astrocytes and neurons are varying degrees of damage, open the tight junctions between endothelial cells, the dissolution of the basement membrane, cell edema more serious. Borneol improve the most prominent role, no abnormal changes in the basic structure of the three cells; musk improvement mainly reflected in the capillary endothelial cells and astrocytes, but neurons of the uneven distribution of the electron cloud; Styrax followed show some improvement role benzoin role is not obvious. Musk can significantly reduce ischemic brain tissue VEGF levels, reduce the trend of MMP-9; borneol significantly lower VEGF levels; storax and benzoin VEGF and MMP-9 role is not obvious. ⑤ Compared with the sham group, the rat model of myocardial tissue Na-K-ATPase, Ca2-Mg2-ATPase activity were significantly decreased, borneol could significantly improve the Na-K-ATPase activity in the animal model of myocardial tissue on SOD, MDA Ca2-Mg2-ATPase is not obvious; musk, styrax, benzoin rat model of myocardial tissue Na-K-ATPase, Ca2-Mg2-ATPase, SOD, MDA no significant role. Conclusion: ① musk, borneol, Styrax benzoin four flavor drug has a certain open effect of the blood-brain barrier in mice physiological state, may play resuscitation Xingshen efficacy of pharmacological basis of one good take channeling these drugs aromatic. Musk, borneol against incomplete cerebral ischemia and reperfusion injury in mice blood-brain barrier permeability abnormal increase, showing a protective effect of the blood-brain barrier function in the pathological state. Styrax benzoin effect was weaker than musk, borneol. Aromatic resuscitation drugs on cerebral ischemia-reperfusion injury of damage the structure of the blood brain barrier in rats has a protective effect, the mechanism may be associated with downregulation of VEGF, MMP-9 levels. The protective effect of these drugs on the pathological state of the blood-brain barrier function may be one of the pharmacological basis for the treatment of ischemic encephalopathy. The results also suggest that the fat-soluble parts of the drug may be the material basis of its role in the brain tissue. ② aromatic resuscitation drugs is a protective effect on cerebral ischemia reperfusion injury in rats, musk, borneol role was excellent, followed by Styrax benzoin weak effects, mechanism of action may be related to anti-oxidation, inhibition of the inflammatory response, improve energy metabolism , anti Ca2 overload and nitric oxide-induced neurotoxicity. 3 the borneol rat model of physiological indicators musk, the Styrax benzoin have differences, which may be related to the potency of the \④ rat model of reperfusion 22h ATPase activity in cardiac tissue significantly lower prompted suture method induced focal cerebral ischemia-reperfusion injury model of energy metabolic pathways may be some damage resulting in myocardial tissue, revealed the heart The link between the brain.
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