Dissertation > Excellent graduate degree dissertation topics show

The Relevance Research of Neuroglobin and Caspase-3 in the Hippocampus CA1 Regin in Global Cerebral Ischemia-reperfused Rats

Author: ChenChangLin
Tutor: ZhangXingYi
School: Zunyi Medical College,
Course: Internal Medicine
Keywords: Cerebral ischemia/reperfusion injury Neuroglobin Caspase-3 Apoptosis
CLC: R741
Type: Master's thesis
Year: 2011
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:To investigate the expression of neuroglobin (Ngb) and Caspase-3 in hippocampus CA1 region of the Sprague-Dawley (SD) rats with global cerebral ischemia/reperfusion(I/R) injury and analyze the expression regularity of Ngb and Caspase-3 in differernt time,meanwhile analyze the relevance of Ngb and Caspase-3 distribution;discuss the effect mechanism of neuroglobin and Caspase-3.provide a reference for clinical.Methods:60 healthy SD rats were randomly divided into 2 groups:sham operation (SO) group and I/R group.each group was assigned into 5 observing time-points: 3,6,12,24,48 hours (h) according to reperfusion time. The SD rats model of global cerebral ischemica/reperfusion was produced by means of simple Pulsinelli-Brierley’s four arteries occlusion method.SO and I/R groups were both intraperitoneally injected with 0.9% Sodium chloride (dose:0.5ml/kg) per six hours after reperfusion.Then SD rats’cerebrums were taken out of their skull at each of observing time-point.The expression activation of Caspase-3 and Ngb protein in pyramidal cells in hippocampus comuammonis (CA)1 region were examined by immunohistochemical method (SABC) and Caspase-3 mRNA and Ngb mRNA by in situ hybridization, measured their average optical density(OD),and hematoxylin and eosin (HE) staining was also performed to detect the number of surviving pyramidal cells,and terminal-deoxynucleotidy transferase medatd dUTP nick end labeling (TUNEL) was used to detect apoptotic pyramidal cells (positive cells), And calculated the apoptotic rate of cells.Results:SO group,the expression of average optical density (OD) of Caspase-3 protein and Caspase-3 mRNA,Ngb and Ngb mRNA were not statistically significant(P>0.05);the number of surviving neurons and apoptotic rate of cells was no significant changes,there was no statistically significant (P>0.05);In the I/R group, Caspase-3 protein and Caspase-3 mRNA began to express at 3h after reperfusion,at the top at 24h,decreased gradually after 48h,24h has variability conspicuous statistically significant compared with3、6、12、48h (P<0.05);Ngb protein and Ngb mRNA’s dispersively expressed at 3h,6h after reperfusion,3、12、24、48h compared with the SO group was statistically significant(p<0.05);the number of surviving neurons and apoptotic rate of cells was significant changes,there was statistically significant (P<0.05).In SO group,Caspase-3 compared with the Ngb was not statistically significant(P>0.05). In the I/R group was statistically significant(P<0.05). Correlated analysis showed that there was negative correlation between the expression levels of Ngb and Caspase-3(r=-0.882,p<0.05),Ngb and apoptotic rate of cells(r =-0.984, P<0.01);positive correlation between Caspase-3 and apoptotic rate of cells (r =0.994, P<0.01).Conclusion:(1) After cerebral ischemia-reperfusion injury Caspase-3’expression was increased with time,meanwhile Ngb was reduced; (2) Caspase-3 has a negative correlation with the Ngb after global cerebal ischemia/reperfusion injury,neuroglobin may inhibit neuronal apoptosis.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Neurology and psychiatry > Neurology
© 2012 www.DissertationTopic.Net  Mobile