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Effective Substances Research of Chinese Multiherb Medicine Wen-Luo-Fang with HPLC-DAD-MS~n

Author: ZhangYiXuan
Tutor: ShiZuo
School: Beijing Union Medical College
Course: Pharmacognosy
Keywords: Wen-Luo-Fang Selaginella tamariscina Atractylodes macrocephala quantitive qualitative HPLC-DAD-MS~n
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 298
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Abstract


Treatment with traditional Chinese medicinal composite is one of the most important characteristics of traditional Chinese medicine (TCM). Studying material basis of TCM composite contributes a lot to modernizing the industry of Chinese medicinal herbs. In the present study, Wen-Luo-Fang, a multiherb medicine used to treat Rheumatoid Arthritis, is employed to address this key point. This dissertation is divided into four parts to study the effective substances of Chinese multiherb medicine Wen-Luo-Fang.Part I:Summary of the related literatureThe chemical constituents of Atractylodes macrocephala Kodiz, Aconitum carmichaeli Debx., Cinnamonun cassia Presl and Selaginella tamariscina (Beuav.) Spring which constitute Wen-Luo-Fang were summed up.66 references were cited.Part II:Studies on the the components of Selaginella tamariscina (Beuav.) Spring by using HPLC-DAD-MS"Biflavonoids are the main bioactive constituents of Selaginella tamariscina (Beuav.) Spring but few studies have reported the structural characterization method of biflavonoids with HPLC-MS". Firstly, The HPLC-DAD-MSn was used to study the fragmentation behavior of three main types of biflavonoids using seven biflavonoid reference compounds. And 17 biflavonoids were identified in an extract of Selaginella tamariscina based on the fragmentation behavior of different types of biflavonoids,5 of which have not been previously reported as the constituents of this plant. This result provides a fast and powerful approach for the online structural elucidation of biflavonoids from Selaginella plants and prescription Wen-Luo-Fang.PartⅢ:Studies on the the components of Atractylodes macrocephala Kodiz by using HPLC-DAD-MSnThe HPLC-DAD-MSn method was developed to study the sesquiterpenes and polyacetylenes from Atractylodes macrocephala Kodiz. AtractylenolideⅠ,ⅡandⅢwere used to study the fragmentation behavior of sesquiterpenes by HRMS firstly, and 9 sesquiterpenes were tentatively characterized based on their UV and MS spectra. Furthermore,9 polyacetylene compounds were identified by HPLC-MSn for the first time, 3 of which have not been previously reported as the constituent of this plant. The structural types of the other 16 compounds were also tentatively identified. This result makes it easier to analyze the effective substances in Wen-Luo-Fang and other plants from Atractylodes DC.Part VI:Effective substances research of Chinese multiherb medicine Wen-Luo-Fang with HPLC-DAD-MS"1 Qualitative analysis of the main constituents of Wen-Luo-FangThe main constituents in Wen-Luo-Fang were identified by coupling technique HPLC-DAD-MS". A total of 44 compounds including aconitine-type alkaloids, sesquiterpenes, biflavonoids and other constituents were identified in Wen-Luo-Fang. The results lay a good foundation for further serum pharmacochemistry study and quantitative analysis of the major substances in Wen-Luo-Fang.2 Initial studies on the constituents absorbed into blood after the administration of of Wen-Luo-FangHPLC-DAD-MS" method was employed in the analysis of the constituents absorbed into blood after the oral administration of Wen-Luo-Fang. After comparing the HPLC-MS information of blank plasma and Wen-Luo-Fang extract,10 original compounds of Wen-Luo-Fang were observed in the plasma including 4-benzoylmesaconine, mesaconitine, hypaconitine, hypaconitine, coumarin,2-hydroxy cinnamaldehyde, cinnamic acid, amentoflavone, atractylenolide I and atractylenolide III. The content of compounds from Cinnamonun cassia Presl and Atractylodes macrocephala Kodiz are higher than the others.3 Quantitative determination of eight main constituents of Wen-Luo-FangAccording to the result above, a UPLC-DAD method for quantitative determination of 8 main effective substances which were the main constituents of the formula(including 5 compounds detected in serum) was established. The methord of quantitative determination of 8 major components in Wen-Luo-Fang not only elevates the quality control standard of the formula but also makes the clinic application of Wen-Luo-Fang more effective and reliable.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
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