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Tibetan medicine Lu the heap Dorje water extract on the reproductive system of male rats and the study of the mechanism of action

Author: HuHuaGang
Tutor: XuSiFan
School: Central University for Nationalities
Course: National Medicine
Keywords: Codonpsis thalictrifolia Wall. testosterone Leydig cells cAMP
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 1
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Abstract


Objective:Human prosperity is based on reproduction. Reproductive health is the core of human health. In recent years, the incidence of diseases on male reproductive system is increasing year by year, so male reproductive health issue is concerned increasingly which is now the focus of medical research. Tibetan medicine has a long history about several thousand years. It has a complete theory about it’s traditional medicine. It will be significant for human reproductive health to find a nature and effective drug from tibetan medicine.The research object is Codonopsis thalictrifolia Wall.. The purpose of research is to observe and test the effects of water extraction from Codonpsis thalictrifolia Wall.on the reproductive system of infant and adult rats. The effect of rats serum of given drug group on the testosterone secretion of Leydig cells and its mechanism were researched by the method of Serum pharmacology. This study will provide scientific datas for the exploitation of Codonpsis thalictrifolia Wall.Method:Infant rats and adult rats were administrated with water extraction of Codonpsis thalictrifolia Wall.or Codonopsis pilosula Nannf.. After 2 weeks, rats’ weight, density of sperm, testis weight, epididymis, and spermatophore were tested. Testosterone of rats were tested by radioimmunoassay. DHT, FSH, and LH were tested by ELISA. The differences between rats serum of given drug group and control group were analyzed by HPLC, and also the differences between water extraction of Codonopsis thalictrifolia Wall, and Codonopsis pilosula Nannf..The culture method of Leydig cell in vitro was established. The effect of rats serum of given drug group on testosterone secretion of Leydig cells and its mechanism were researched by the method of Serum pharmacology.Result:After having been fed the water extract from Codonopsis thalictrifolia Wall, or Codonopsis pilosula Nannf. for 14 days, it can be found that testis weight, sperm density, and testosterone content in serum have been reduced at dose-dependent in infant rats(P<0.05). The content of DHT in serum has been significantly decreased at dose of 10g/Kg(P<0.05), and the weight increase of infant rats can be significantly decreased at dose of 40g/Kg(P<0.01). At the same time the content of LH and FSH in serum have been significantly increased at dose-dependent in infant rats(P<0.05).After having been fed the water extract from Codonopsis thalictrifolia Wall.and Codonopsis pilosula Nannf. for 14 days, it can be found that the body weight increased(P<0.05); the weight of testis, epididymis and seminal vesicle were decreased(P<0.05); the sperm density, the content of testosterone and DHT in serum reduced significantly at dose-dependent in adult rats(P<0.05). At the same time the content of LH and FSH in serum increased at dose-dependent in adult rats.Compared with the serum chromatograms of control group with HPLC, there is an obvious peak at 20 minutes in 280nm on serum chromatograms of Codonopsis thalictrifolia Wall.or Codonopsis pilosula Nannf. groups respectively. It indicates that the serum of rats contains efficacy substance.Compared with the rats serum chromatograms given medicine with HPLC, there is not an obvious peak at 20 minutes in 280nm on chromatograms of the water extract from Codonopsis thalictrifolia Wall. and Codonopsis pilosula Nannf. groups respectively. It indicates that the efficacy substances in serum are the metabolites from Codonopsis thalictrifolia Wall.or Codonopsis pilosula Nannf..The isolated Leydig cells motility rate was confirmed by trypan blue exclusion experiment. Trypan blue exclusion experiment results showed that cells’ motility rate was up to 95% in 24h in vitro.The testosterone secretion of Leydig cells gradually reduce along with the increase in volume of the serum which contained drug. Compared with control group, when the volume of Codonopsis thalictrifolia Wall. serum is more than 80μl, the testosterone secretion and the content of cAMP in Leydig cells decrease significantly (p<0.05). When the volume of Codonopsis pilosula Nannf. serum is more than 40μl, the testosterone secretion and the content of cAMP in Leydig cells are lower than control group(p<0.05).Conclusions:The efficacy substances in serum are the metabolites from Codonopsis thalictrifolia Wall. or Codonopsis pilosula Nannf.Through the cAMP pathway, the efficacy substance in serum could reduce testosterone secretion of Leydig cells.Codonopsis thalictrifolia Wall.and Codonopsis pilosula Nannf. could restrain the growth and development of the reproductive system. It indicates that we should be carefully use them alone or at high dose. It’s better use them compatibly with other herbal medicines.

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