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Analytical Investigations on the Antioxidant Activities of Radix Astragali

Author: ShiNi
Tutor: SuXiaoLi
School: Hunan Normal University
Course: Analytical Chemistry
Keywords: Antioxidant Spectrophotometry Flow injection chemiluminescence Current biosensor Astragalus Honey roast
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 244
Quote: 1
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Abstract


Astragalus is blindly common traditional Chinese medicine, the main chemical components such as phenolic acids, polysaccharides, saponins, flavonoids and amino acids, improve immune function and memory, anti-bacterial anti-inflammatory, anti-oxidation, anti-radiation, anti-cancer, protect the heart and brain blood vessels and brain cells and so on. The honey roast law Astragalus most commonly used processing methods, but also one of the most commonly used traditional Chinese medicine processing methods, synergies and lowering the efficacy of low toxicity. Astragalus the effectiveness of its anti-oxidative action. At present, the antioxidant activity of astragalus and other traditional Chinese methods for the detection electron spin resonance, chemiluminescence, spectrophotometry, high performance liquid chromatography - electrospray ionization - mass spectrometry, capillary electrophoresis - chemiluminescence method. Astragalus antioxidant active ingredient is more complex, and its mechanism of action are not the same. Therefore, you can not use a single indicator or method to be evaluated. Moreover, the current research about the honey roast Astragalus antioxidant activity seldom. In view of this, the paper Astragalus study, antioxidant effects from traditional Chinese medicine point of view of its material basis for the analysis their honey roast efficiency mechanism. The details are as follows: (1) the pharmacological effects of Astragalus and antioxidant activity detection method research progress, and discusses its detection principle. Astragalus and honey roast Astragalus, water extract and alcohol extract total phenolic content was determined using the Folin-Ciocalteu method, as well as the spectrophotometric determination of extract of astragalus and honey roast Astragalus of the superoxide anion radical, hydroxyl radical and DPPH radical scavenging capacity, a comparative analysis of the impact of the honey roast of Astragalus anti-oxidation. The results show that the astragalus and honey roast Astragalus extract has antioxidant properties, which the alcohol extract the most liquid in the highest total phenolic content and antioxidant capacity. Established antioxidant activity detection for the determination of astragalus and honey roast Astragalus flow injection chemiluminescence method. The results showed that the extract of astragalus and honey roast Astragalus of chemiluminescence was inhibited by which of honey roast Astragalus alcohol extract the strongest rejection. Developed based on the the SOD enzyme electrode current biosensor for detecting Astragalus antioxidant properties. Modified electrodes were characterized by cyclic voltammetry. Under optimized conditions to determine the difference in the oxidation current of honey roast law Astragalus antioxidant properties. Experimental results show that astragalus and honey roast Astragalus extract has antioxidant properties.

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