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Extract and Study of the Chemical Constituents from Pomegranate in Xinjiang

Author: WuJia
Tutor: JieChengXi
School: Xinjiang University
Course: Analytical Chemistry
Keywords: Uygur medicine Pomegranate Chemical Constituents antioxidation activity
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 126
Quote: 1
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Abstract


Pomegranate is a sort of fruit that is liked by all the people in Xinjiang. Pomegranate Peel and Pomegranate Seed were Uyghur medicine of common medicinal materials. This paper researched extraction of effective chemical composition (flavonoid, polysaccharides, alkaloid, fatty acids and trace elements) and analyzed chemical composition in Pomegranate. And the main research content and results were as follows:1. Total flavonoids from Pomegranate Peel were extracted and determined. (1) Study reflux extraction and ultrasonic extraction technologies of total flavonoids from Pomegranate Peel, and the best extracting technology was established by single factor and orthogonal experiment. The content of total flavonoids was determined by uv-vis spectrophotometry. Under this optimized conditions, the average percent of reflux extraction was 95.1 mg/g, the average percent of ultrasonic extraction was 84.8 mg/g. (2) HPLC-MS/n was used to analyzed some compounds from the methanol extract of Pomegranate Peel, and rutin was identified in the six compounds. (3) The content of Rutin in Pomegranate Peel was detected by HPLC. (4) Studies on inhibition on nitrosation and antioxidation activity of total flavonoids in Pomegranate Peel.2. Polysaccharides in Pomegranate Peel was extracted, purified and determined. (1) By phenol-H2SO4-coloration method, the content of polysaccharides in Pomegranate Peel was determined. And the average rate of extraction was 12.7%. (2) Studies on inhibition on nitrosation and antioxidation activity of polysaccharides from Pomegranate Peel.3. Study optimized extraction technology of total alkaloids from Pomegranate Peel, and determination the content of total alkaloids in Pomegranate Peel. By using acid dye colorimetry as the analytical tool and orthogonal test, the highest extraction yield can be obtained when the ammonium sulfate was 8.0 g, the volume of propanol was 20 mL, the ratio of alcohol to water was 0.8:1 for the water of aqueous two-phase system, and the time of ultrasonic was 60min. Under this optimized conditions, the extraction percent of total alkaloids from Pomegranate Peel was 0.559 mg/g.4. The fatty acids were extracted from pomegranate seed was analyzed by GC-MS. 10 kinds of chemical compounds were separated, and 6 kinds of them were identified. And Pomegranate seed content in the most fatty acid components was 9,12,15-Octadecatrienoic acid(76.06%). The trace elements of K, Ca, Mg, Fe, Al, Cu, Zn, Mn, Na, Li, P, S in the Pomegranate Peel and Pomegranate Seed were determination by inductively coupled plasma atomic emission spectrometry (ICP-AES) with microwave digestion. Different concentrations of the ethanol extract from Pomegranate Peel, the ethanol extract from Pomegranate Seed and the alkaloids from Pomegranate Peel were used as antimicrobial agents. The antimicrobial activity on four species of bacteria and the minimal inhibition concentrations (MIC) were analyzed.

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