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In this study, Cymbidium, Cymbidium, cold blue, Chunlan and gold prodigy, Phalaenopsis and Cattleya objects using solid phase micro extraction (SPME) and simultaneous distillation extraction (SDE) to collect orchids volatile ingredients, combined gas chromatography - mass spectrometry (GC-MS) orchids volatile components were measured, in the use of computers to retrieve, the artificial spectrum analysis technology and access to the extensive literature on the basis of qualitative and quantitative study of orchids volatile components. Determine the the China Orchid volatile constituents of sample pre-treatment methods; initially identified the the China Orchid characteristic aroma components. Comparative analysis of the difference between the volatile components of different colors Cymbidium; cold blue and Chunlan floral volatile components, portfolio and orchids Cymbidium SPME sample pre-treatment conditions Faxiang site. Results of further study Chinese orchid aroma components, in-depth development and utilization of Chinese orchid resources to lay a solid foundation, and provide some theoretical basis for quality evaluation of Chinese orchid aroma. Main conclusions: 1. By relatively solid phase micro extraction technology and the distillation extraction the two aroma collect technical Cymbidium volatile components, determine the solid-phase micro-extraction technology as a new type of aroma components collection methods is better than at the same time distillation extraction method. Initially identified 3 - oxo oxo-2 - (2 - pentynyl yl) - cyclopentyl-methyl acetate, trans -3 - oxo-2 - (cis-2 - pentene-yl) cyclopentyl methyl acetate, six-enoate and methyl jasmonate 4,7,10,13,16,19 - twenty-two by carbon for Orchid characteristic floral ingredients. 3 different colors Cymbidium were found in 84 kinds of volatile components, a total of components 33 kinds, benzoic aldehyde the three suits Cymbidium common fragrance ingredient, methyl salicylate, trans - nerolidol, Orange Flower tertiary alcohol, linalool, isobutyl loose Camphor, pine carvone alcohol, geraniol, trans -3 - oxo-2 - (cis-2 - pentene-yl) - cyclopentyl methyl acetate and the farnesol. Different the suit kanran of detecting volatile components of 29 kinds, of two kinds suit kanran Total aroma components Titanium farnesol and (E)-β-farnesene, farnesol alkylene, 2,5 - octadecadienoic two ynoate, 4,7,10,13,16,19 - 22 carbon hexaene, methyl trans -3 - oxo-2 - (cis-2 - pentene-yl) - cyclopentyl acetate methyl ester, 3 - oxygen-oxo-2 - (2 - pentynyl) - cyclopentyl-methyl acetate and methyl jasmonate. The different colors Chunlan were found in 56 kinds of volatile components, two kinds of color Chunlan total of 18 kinds of detection ingredients that the largest contribution, followed by terpenes alcohols Chunlan floral. 4 a Hong unscented kanran analysis identified 55 kinds of volatile components that farnesol, ylang-ene, (E)-β-farnesene, ocimene, cadinene, farnesene, α - Faber ene, leaves pinene, trans -3 - oxo-2 - (cis-2 - pentene-yl) - cyclopentyl methyl acetate, 3 - oxo-2 - (2 - pentynyl) - cyclopentyl acetate, 4,7,10,13,16,19 - twenty-two by Methyl squalene, methyl jasmonate, methyl jasmonate, 2,5 - 18 carbon diyne acid methyl acacia alcohol acetate ester of cold blue floral important contribution. Hong unscented Chunlan analysis identified a total of 73 kinds of volatile components that sandalwood alcohol, not orange ene oxide the 4,5 - dehydrogenation - isolongifolene, and cis - bisabolene epoxide, gold leucocephala aldehyde, 3 - oxo-2 - (2 - pentyn-yl) - cyclopentyl methyl acetate, trans -3 - oxo-2 - (cis-2 - pentene-yl) - cyclopentyl methyl acetate, 4,7,10,13,16,19 - docosahexaenoic allyl methyl orange oil, cis - Australia sandalwood alcohol, methyl jasmonate ionone and leaves ene Chunlan floral . Fresh weight 0.6g, extraction time of 60 min handle scale to 4cm, desorption temperature of 250 ° C, extraction the fiber using 85μmPA best combination of Cymbidium SPME sample preparation conditions. 6. Comparative analysis of the Cymbidium cold blue flowers in different parts of the volatile components, petal perfumed orchids main parts.
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